Interplay between Jasmonic Acid, Phosphate Signaling and the Regulation of Glycerolipid Homeostasis in Arabidopsis
Interplay between Jasmonic Acid, Phosphate Signaling and the Regulation of Glycerolipid Homeostasis in Arabidopsis
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DOI：
10.1093/pcp/pcz027
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发表时间：
2019-06-01
影响因子：
4.9
通讯作者：
Marechal, Eric
中科院分区：
文献类型：
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作者：
Chevalier, Florian;Cuyas, Laura;Marechal, Eric
在缺乏磷酸盐 (Pi) 的条件下培养的拟南芥中，茉莉酸 (JA) 生物合成和信号传导被激活。这种激活主要发生在光合组织中，在根中不太重要。在叶子中，JA 生物合成的增强与 Pi 缺乏引发的膜甘油脂重塑同时发生。我们探讨了 JA 对 Pi 剥夺和补给反应中甘油脂重塑的动力学和程度的可能作用。基于对野生型拟南芥和 coi1-16 突变体中基因表达、JA 生物合成和甘油脂重塑的综合分析，JA 信号传导似乎在确定磷脂酰胆碱、磷脂酸 ( PA)、单半乳糖二酰甘油 (MGDG) 和二半乳糖二酰甘油的基础水平中很重要。 JA 对 MGDG 稳态水平和波动的影响似乎是矛盾的。在 coi1-16 突变体中，MGDG 的稳态水平较高，可能是由于突变体中 PA 水平较高，激活了 MGD1，并且 MGD3 表达增加。这些结果支持 JA 在限制这种脂质的总体含量方面可能产生的影响。关于血脂变化，Pi 剥夺后，JA 似乎与特定的 MGDG 增加相关。 Pi 重新供应后，虽然甘油脂重塑基因的表达恢复到基础水平，但 JA 生物合成和信号基因仍然上调，可能是由于 JA 诱导的正反馈仍然活跃。对合成 MGDG 的酶的独特影响，即下调 MGD3，可能激活 MGD1 表达并通过 PA 限制 MGD1 的激活，可能使 JA 在半乳糖脂变化的复杂微调中发挥作用。
Jasmonic acid ( JA) biosynthesis and signaling are activated in Arabidopsis cultivated in phosphate ( Pi) deprived conditions. This activation occurs mainly in photosynthetic tissues and is less important in roots. In leaves, the enhanced biosynthesis of JA coincides with membrane glycerolipid remodeling triggered by the lack of Pi. We addressed the possible role of JA on the dynamics and magnitude of glycerolipid remodeling in response to Pi deprivation and resupply. Based on combined analyses of gene expression, JA biosynthesis and glycerolipid remodeling in wild-type Arabidopsis and in the coi1-16 mutant, JA signaling seems important in the determination of the basal levels of phosphatidylcholine, phosphatidic acid ( PA), monogalactosyldiacylglycerol ( MGDG) and digalactosyldiacylglycerol. JA impact on MGDG steady state level and fluctuations seem contradictory. In the coi1-16 mutant, the steady state level of MGDG is higher, possibly due to a higher level of PA in the mutant, activating MGD1, and to an increased expression of MGD3. These results support a possible impact of JA in limiting the overall content of this lipid. Concerning lipid variations, upon Pi deprivation, JA seems rather associated with a specific MGDG increase. Following Pi resupply, whereas the expression of glycerolipid remodeling genes returns to basal level, JA biosynthesis and signaling genes are still upregulated, likely due to a JA-induced positive feedback remaining active. Distinct impacts on enzymes synthesizing MGDG, that is, downregulating MGD3, possibly activating MGD1 expression and limiting the activation of MGD1 via PA, might allow JA playing a role in a sophisticated fine tuning of galactolipid variations.