Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs
Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs
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DOI：
10.1007/s001220051017
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发表时间：
1998-12-01
影响因子：
5.4
通讯作者：
Motto, M
中科院分区：
文献类型：
--
作者：
Pejic, I;Ajmone-Marsan, P;Motto, M
基于DNA的指纹技术已被证明在遗传相似性研究中是有用的。RFLP仍然是最常用的估计植物物种的遗传多样性，但最近开发的基于PCR的标记技术，RAPD，SSR和AFLP，在这些调查中发挥着越来越重要的作用。使用一组33个玉米自交系，我们报告的技术比较，以评估其信息量和遗传多样性研究的适用性。四种检测方法检测到的多态性数量不同。以预期杂合度和平均等位基因数来衡量的信息含量，SSR的信息含量较高，而AFLP的多态性水平最低。然而，AFLP是最有效的标记系统，因为它们能够在一次扩增中显示多个条带。事实上，与其他方法相比，AFLP的测定效率指数高出十倍以上。除RAPD外，遗传相似性树高度相关。SSR和AFLP技术可以代替RFLP标记进行遗传相似性研究，因为它们在对系谱选择的自交系进行基因分型时具有相当的准确性。Bootstrap分析表明，在一组分析的线，使用的标记的数量是足够的一个可靠的估计遗传相似性和标记技术的有意义的比较。
DNA-based fingerprinting technologies have proven useful in genetic similarity studies. RFLP is still most commonly used in the estimation of genetic diversity in plant species, but the recently developed PCR-based marker techniques, RAPDs, SSRs and AFLPs, are playing an increasingly important role in these investigations. Using a set of 33 maize inbred lines we report on a comparison of techniques to evaluate their informativeness and applicability for the study of genetic diversity. The four assays differed in the amount of polymorphism detected. The information content, measured by the expected heterozygosity and the average number of alleles, was higher for SSRs, while the lowest level of polymorphism was obtained with AFLPs. However, AFLPs were the most efficient marker system because of their capacity to reveal several bands in a single amplification. In fact, the assay efficiency index was more than ten-fold higher for AFLPs compared to the other methods. Except for RAPDs, the genetic similarity trees were highly correlated. SSR and AFLP technologies can replace RFLP marker in genetic similarity studies because of their comparable accuracy in genotyping inbred lines selected by pedigree. Bootstrap analysis revealed that, in the set of lines analysed, the number of markers used was sufficient for a reliable estimation of genetic similarity and for a meaningful comparison of marker technologies.