Role of tryptophan-metabolizing microbiota in mice diarrhea caused by Folium sennae extracts
Role of tryptophan-metabolizing microbiota in mice diarrhea caused by Folium sennae extracts
复制标题
色氨酸代谢微生物群在番泻叶提取物引起的小鼠腹泻中的作用
DOI：
10.1186/s12879-020-4794-5
复制
发表时间：
2020-06-29
期刊：
影响因子：
4.2
通讯作者：
Tan, Zhoujin
中科院分区：
文献类型：
--
作者：
Zhang, Chenyang;Shao, Haoqing;Tan, Zhoujin
研究背景虽然有报道表明，腹泻病可导致肠道菌群多样性紊乱，但肠道菌群特征及具体机制尚不清楚。本研究的目的是探讨党参引起腹泻的机制，其与肠道细菌特性重塑和代谢异常有关。结果党参提取物干预后，Chao 1指数和Shannon指数均显著降低。苦参提取物干预组小鼠的β多样性聚类与对照组明显分离。结合PPI网络分析，细胞色素P450酶代谢是丹参引起腹泻的主要信号通路。10个细菌植物群群落在干预前后差异有统计学意义：副雷沃氏菌属、链球菌属、浮鱼属、萨特氏菌属和支原体的丰度显著增加;阿得克罗伊特氏菌属、乳酸杆菌属、脱盐杆菌属、多雷亚氏菌属和颤螺旋菌属的丰度显著降低。10个肠道菌群中有7个与色氨酸衍生物的合成有关，影响氨基色氨酸向L-色氨酸的转化，导致宿主色氨酸代谢异常。结论人参皂苷靶向细胞色素P450 3A 4改变肠道菌群的特性，干预肠道菌群如链球菌、Sutterella和Dorea的色氨酸代谢。这可能是党参提取物致腹泻的肠道微生态机制。
BackgroundAlthough reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality.ResultsFor the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased. The Beta diversity clusters of mice interfered by Folium sennae extracts were distinctly separated from control group. Combining PPI network analysis, cytochrome P450 enzymes metabolism was the main signaling pathway of diarrhea caused by Folium sennae. Moreover, 10 bacterial flora communities had statistical significant difference with Folium sennae intervention: the abundance of Paraprevotella, Streptococcus, Epulopiscium, Sutterella and Mycoplasma increased significantly; and the abundance of Adlercreutzia, Lactobacillus, Dehalobacterium, Dorea and Oscillospira reduced significantly. Seven of the 10 intestinal microbiota communities were related to the synthesis of tryptophan derivatives, which affected the transformation of aminotryptophan into L-tryptophan, leading to abnormal tryptophan metabolism in the host.ConclusionsFolium sennae targeted cytochrome P450 3A4 to alter intestinal bacterial characteristic and intervene the tryptophan metabolism of intestinal microbiota, such as Streptococcus, Sutterella and Dorea, which could be the intestinal microecological mechanism of diarrhea caused by Folium sennae extracts.