Development of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for analysing nobiletin in citrus and herb samples
Development of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for analysing nobiletin in citrus and herb samples
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DOI：
10.1016/j.foodchem.2019.04.101
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发表时间：
2019-09-30
期刊：
影响因子：
8.8
通讯作者：
Jiao, Bining
中科院分区：
文献类型：
--
作者：
Cui, Yongliang;Zhao, Jing;Jiao, Bining
Nobiletin是一种主要存在于柑橘类水果中的多甲氧基黄酮，据报道对人类健康具有多种有益的生物活性。它是中药陈皮和枳壳中的重要活性成分。为检测柑橘和中草药中的川陈皮素含量，建立了一种基于单克隆抗体的间接竞争酶联免疫吸附试验（icELISA）。其中位抑制浓度（IC 50）为2.43 +/- 0.19 ng/mL，工作范围为0.52-12.3 ng/mL。柑橘皮、果肉和果汁样品的平均回收率为72.5-85.3%。此外，该方法还对11个柑橘品种样品和4个草药样品进行了检测。采用超高效液相色谱-串联质谱法（UPLC-MS）对不同柑橘品种和药材样品中的川陈皮素含量进行了分析。结果表明，所建立的免疫分析方法适用于柑橘和中草药样品中川陈皮素的检测。
Nobiletin, a polymethoxyflavone mainly found in citrus fruits, have been reported to exhibit various beneficial biological activities for human health. It is an important bioactive compound in traditional Chinese medicine, Pericarpium Citri Reticulatae and Fructus Aurantii. To detect the contents of nobiletin in citrus and herb samples, we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies. It possessed a median inhibition concentration (IC50) of 2.43 +/- 0.19 ng/mL and a working range of 0.52-12.3 ng/mL. The assay exhibited the average recoveries of 72.5-85.3% in citrus peel, pulp and juice samples. Moreover, eleven citrus cultivars samples and four herb samples were also detected by the icELISA. The nobiletin content varied in different citrus cultivars samples and herb samples, which were confirmed by the ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS). These results indicated that the developed immunoassay was suitable for detecting nobiletin in citrus and herb samples.