In vitro selection of histone H4 aptamers for recognition imaging microscopy
In vitro selection of histone H4 aptamers for recognition imaging microscopy
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DOI：
10.1021/ja076488m
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发表时间：
2007-11-28
影响因子：
15
通讯作者：
Chaput, John C.
中科院分区：
文献类型：
--
作者：
Lin, Liyun;Hom, Doris;Chaput, John C.
识别成像显微镜是一种分析技术，用于绘制复杂生物样品中存在的特定蛋白质分子的形貌和化学身份。该技术依赖于将抗体拴在AFM探针的悬臂尖端上，以检测沉积在云母表面上的同源抗原。尽管这项技术具有分辨纳米级间距的单个分子的能力，但识别步骤仍然受到合适质量抗体的可用性的限制。在这里，我们报告了在体外选择和识别成像的抗组蛋白H4适体。除了识别适体的高度碱性蛋白质，这些结果表明，适体提供了一个有效的，具有成本效益的路线，高度选择性的亲和试剂识别成像显微镜。
Recognition imaging microscopy is an analytical technique used to map the topography and chemical identity of specific protein molecules present in complex biological samples. The technique relies on the use of antibodies tethered to the cantilever tip of an AFM probe to detect cognate antigens deposited onto a mica surface. Despite the power of this technique to resolve single molecules with nanometer-scale spacing, the recognition step remains limited by the availability of suitable quality antibodies. Here we report the in vitro selection and recognition imaging of anti-histone H4 aptamers. In addition to identifying aptamers to highly basic proteins, these results suggest that aptamers provide an efficient, cost-effective route to highly selective affinity reagents for recognition imaging microscopy.