Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH
Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH
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DOI：
10.1038/s41598-019-46197-6
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发表时间：
2019-07-04
影响因子：
4.6
通讯作者：
Li, Lihui
中科院分区：
文献类型：
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作者：
Han, Haiming;Liu, Weihua;Li, Lihui
冰草Agropyron cristatum（L.）Gaertn.（P基因组）作为牧草栽培，可以为小麦的改良提供许多有用的基因。随着基因组学和荧光原位杂交（FISH）技术的发展，用于鉴定植物染色体的探针也不断发展。然而，关于A.嵴状染色体在此，具有重复序列pAcTRT 1和pAcpCR 2的FISH使得能够鉴定所有二倍体A。嵴状染色体本文对A.根据5个二倍体A. cristatum个体。同源染色体的结构多态性不仅在不同个体之间观察到，而且在个体内部也观察到。此外，17个小麦-A。用pAcTRT 1和pAcpCR 2探针对含有不同P基因组染色体的cristatum渐渗系进行了鉴定。二倍体A. cristatum是通过鉴定这些遗传鉴定的渐渗系中的P染色体与二倍体A.嵴状染色体这两个探针对四倍体A的所有染色体也都有效。cristatum，以及两个四倍体A. cristatum材料与二倍体A.鸡冠这些结果为P染色体组的染色体鉴定和系统发育研究提供了有效的手段。
Agropyron cristatum (L.) Gaertn. (P genome) is cultivated as pasture fodder and can provide many desirable genes for wheat improvement. With the development of genomics and fluorescence in situ hybridization (FISH) technology, probes for identifying plant chromosomes were also developed. However, there are few reports on A. cristatum chromosomes. Here, FISH with the repeated sequences pAcTRT1 and pAcpCR2 enabled the identification of all diploid A. cristatum chromosomes. An integrated idiogram of A. cristatum chromosomes was constructed based on the FISH patterns of five diploid A. cristatum individuals. Structural polymorphisms of homologous chromosomes were observed not only among different individuals but also within individuals. Moreover, seventeen wheat-A. cristatum introgression lines containing different P genome chromosomes were identified with pAcTRT1 and pAcpCR2 probes. The arrangement of chromosomes in diploid A. cristatum was determined by identifying correspondence between the P chromosomes in these genetically identified introgression lines and diploid A. cristatum chromosomes. The two probes were also effective for discriminating all chromosomes of tetraploid A. cristatum, and the differences between two tetraploid A. cristatum accessions were similar to the polymorphisms among individuals of diploid A. cristatum. Collectively, the results provide an effective means for chromosome identification and phylogenetic studies of P genome chromosomes.