Keratinocyte and epidermal leukocyte expression of CD36 (OKM5) in benign and malignant skin diseases.
Keratinocyte and epidermal leukocyte expression of CD36 (OKM5) in benign and malignant skin diseases.
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良性和恶性皮肤病中角质形成细胞和表皮白细胞 CD36 (OKM5) 的表达。
DOI：
10.2340/00015555701822
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发表时间：
1990
影响因子：
3.6
通讯作者：
G. Vejlsgaard
中科院分区：
文献类型：
--
作者：
S. Lisby;E. Ralfkiaer;E. R. Hansen;G. Vejlsgaard
CD 36识别一种88 Kd的糖蛋白，在参与免疫调节的不同细胞群上发现，并通过用γ-干扰素体外处理在角质形成细胞上诱导。因此，我们从48名患有各种皮肤病的患者和5名健康志愿者获得皮肤活检组织，并使用三阶段免疫过氧化物酶法用单克隆抗体OKM 5（CD 36）、抗HLA-DR和OKT 6（CD 1a）对这些进行染色。在正常皮肤中，未观察到CD 36表达。相比之下，患病皮肤中的角质形成细胞为CD 36+。在大多数情况下，染色仅限于颗粒层和棘层，但在银屑病、鳞状细胞癌和淋巴瘤样丘疹病中，可见更广泛的角质形成细胞染色。此外，在过敏性斑贴试验浸润和蕈样肉芽肿中发现了CD 36+表皮白细胞。在广谱皮肤病中表皮细胞表达CD 36的发现表明这些细胞在调节皮肤免疫反应中的作用。
CD36 recognizes a 88 Kd glycoprotein, found on different cell populations involved in immunoregulation and are induced on keratinocytes by in vitro treatment with gamma-interferon. Therefore, we obtained skin biopsies from 48 patients with various dermatological diseases and from 5 healthy volunteers and stained these with monoclonal antibodies OKM5 (CD36), anti-HLA-DR and OKT6 (CD1a) using a three stage immunoperoxidase method. In normal skin, CD36 expression was not observed. In contrast, keratinocytes in diseased skin were CD36+. In most cases, the staining was restricted to the stratum granulosum and the stratum spinosum, but in psoriasis, squamous cell carcinoma and lymphomatoid papulosis, more extensive staining of keratinocytes was seen. In addition, CD36+ epidermal leukocytes were found in allergic patch-test infiltrates and in mycosis fungoides. The findings of CD36 expression by epidermal cells within a broad spectrum of dermatological diseases indicate a role for these cells in the regulation of immune reactions in the skin.