Hemifusion in SNARE-mediated membrane fusion
Hemifusion in SNARE-mediated membrane fusion
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DOI：
10.1038/nsmb921
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发表时间：
2005-05-01
影响因子：
16.8
通讯作者：
Shin, YK
中科院分区：
文献类型：
--
作者：
Xu, YB;Zhang, F;Shin, YK
SNARE 对于细胞内膜融合至关重要。使用 EPR，我们确定了参与酵母运输的囊泡 (v)-SNARE Snc2p 的跨膜结构域 (TMD) 结构。 TMD的结构特征被用来设计v-SNARE突变体，其中大约一半的TMD被删除。当与含有靶膜 (t)-SNARE 的脂质体一起孵育时，含有该突变体的脂质体诱导外叶混合，但不诱导内叶混合。当重组低蛋白浓度时，野生型 SNARE 也检测到半融合。因此，这些结果表明 SNARE 介导的融合可以通过半融合中间体进行转运。
SNAREs are essential for intracellular membrane fusion. Using EPR, we determined the structure of the transmembrane domain (TMD) of the vesicle ( v)-SNARE Snc2p involved in trafficking in yeast. Structural features of the TMD were used to design a v-SNARE mutant in which about half of the TMD was deleted. Liposomes containing this mutant induced outer leaflet mixing but not inner leaflet mixing when incubated with liposomes containing target membrane (t)-SNAREs. Hemifusion was also detected with wildtype SNAREs when low protein concentrations were reconstituted. Thus, these results show that SNARE-mediated fusion can transit through a hemifusion intermediate.