Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis
Cloning and spatiotemporal expression of RIC-8 in Xenopus embryogenesis
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DOI：
10.1016/j.gep.2011.06.001
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发表时间：
2011-10-01
影响因子：
1.2
通讯作者：
Torrejon, M.
中科院分区：
文献类型：
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作者：
Maldonado-Agurto, R.;Toro, G.;Torrejon, M.
RIC-8是促进G蛋白信号传导的高度保守的蛋白质，因为它在G α亚基的子集上充当鸟嘌呤核苷酸交换因子（GEF）。在无脊椎动物中，RIC-8在突触传递和不对称细胞分裂中起着至关重要的作用。作为进一步研究RIC-8在脊椎动物中功能的第一步，我们从热带爪蟾（xtric-8）中克隆了RIC-8基因，并确定了其在整个胚胎发生过程中的时空表达模式。xtric-8转录本在母体和合子中表达，随着发育的进行，它显示出动态表达模式。在早期发育阶段，xtric-8在动物半球中表达，而其表达随后限于神经组织，如神经管和脑，以及眼睛和神经嵴衍生的结构，包括颅面区域的结构。总之，我们的研究结果表明，RIC-8功能与神经系统的发育有关。(C)2011爱思唯尔有限公司版权所有。
RIC-8 is a highly conserved protein that promotes G protein signaling as it acts as a Guanine nucleotide Exchanging Factor (GEF) over a subset of G alpha subunits. In invertebrates, RIC-8 plays crucial roles in synaptic transmission as well as in asymmetric cell division. As a first step to address further studies on RIC-8 function in vertebrates, here we have cloned a ric-8 gene from Xenopus tropicalis (xtric-8) and determined its spatiotemporal expression pattern throughout embryogenesis. The xtric-8 transcript is expressed maternally and zygotically and, as development proceeds, it shows a dynamic expression pattern. At early developmental stages, xtric-8 is expressed in the animal hemisphere, whereas its expression is later restricted to neural tissues, such as the neural tube and the brain, as well as in the eye and neural crest-derived structures, including those of the craniofacial region. Together, our findings suggest that RIC-8 functions are related to the development of the nervous system. (C) 2011 Elsevier B.V. All rights reserved.