Role of NK cells in protection of mice against herpes simplex virus-1 infection.
Role of NK cells in protection of mice against herpes simplex virus-1 infection.
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DOI：
10.4049/jimmunol.138.3.884
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发表时间：
1987-02
影响因子：
4.4
通讯作者：
B. Rager-Zisman;P. Quan;M. Rosner;J. Moller;B. Bloom
中科院分区：
文献类型：
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作者：
B. Rager-Zisman;P. Quan;M. Rosner;J. Moller;B. Bloom
自然杀伤（NK）细胞在体外参与识别和杀死各种病毒感染的靶细胞，但其在体内的作用仍不确定。在这些实验中，研究了NK细胞在调节对单纯疱疹病毒-1（HSV-1）的抗性中的作用。成年C57 BL/6小鼠对HSV-1（HFEM株）具有抗性，但在感染前24小时用环磷酰胺处理后变得高度敏感。在该模型中，被动转移10（8）个正常脾细胞或10（7）个聚I：C处理的脾细胞为72%的受体提供了保护。来自NK细胞缺陷米色小鼠的脾细胞类似地处理未能产生被动保护。负责转移保护的细胞的表型为NK1.1+和去唾液酸GM 1+。NK细胞的转移导致受体肝脏和大脑中HSV滴度的显着降低。这些实验为NK细胞在保护小鼠免受致命性HSV感染的发展中的作用提供了直接证据。
Natural killer (NK) cells have been implicated in the recognition and killing of a variety of virus infected target cells in vitro, yet their role in vivo remains uncertain. In these experiments, the role of NK cells in the regulation of resistance to herpes simplex virus-1 (HSV-1) was studied. Adult C57BL/6 mice are resistant to HSV-1 (HFEM strain), but are rendered highly susceptible by treatment with cyclophosphamide 24 hr prior to infection. In this model, passive transfer of 10(8) normal spleen cells or 10(7) poly I:C-treated spleen cells provided protection for 72% of the recipients. Spleen cells from NK cell-deficient beige mice similarly treated failed to engender passive protection. The phenotype of the cells responsible for transferring protection was NK1.1+, and asialo GM1+. Transfer of NK cells resulted in marked reduction of HSV titers in the livers and brains of recipients. These experiments provide direct evidence for a role for NK cells in protection against development of fatal HSV infection in mice.