Characterization of a di-n-butyl phthalate-degrading bacterial consortium and its application in contaminated soil
Characterization of a di-n-butyl phthalate-degrading bacterial consortium and its application in contaminated soil
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DOI：
10.1007/s11356-018-1862-0
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发表时间：
2018-06-01
影响因子：
5.8
通讯作者：
Lu, Guining
中科院分区：
文献类型：
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作者：
Yang, Jing;Guo, Chuling;Lu, Guining
邻苯二甲酸二丁酯（DBP）是一种增塑剂，在中国被广泛使用，它很容易释放到各种环境中。在本研究中，我们从城市污水处理厂的活性污泥中富集了一个稳定的细菌联合体（B1）。得到的细菌联合体B1具有降解DBP的能力，主要由Pandoraea sp.和Microbacterium sp.组成。在初始浓度为35 ~ 500 mg L-1时，该联合体对DBP的降解效果良好，3 d内降解率达到92%以上。DBP降解的最佳温度为30℃，联合体B1能适应较宽的pH范围（5.5 ~ 8.5）。Illumina测序分析进一步表明，Pandoraea的相对丰度在降解开始时增加，而Microbacterium的相对丰度则减少。在降解后期，潘多拉菌和微菌的相对丰度变化相反。除了DBP，财团B1还可以利用邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙己酯（DEHP）和邻苯二甲酸（PA）作为唯一的碳。此外，在DBP污染土壤中添加B1可以大大提高DBP的去除率，表明B1在DBP污染环境的生物修复中具有很大的潜力。
Dibutyl phthalate (DBP), as a plasticizer, is widely used in China, and it is easily released into diverse environments. In this study, we have obtained a stable bacterial consortium (B1) enriched from municipal sewage treatment plant activated sludge. The obtained bacterial consortium B1 was capable of degrading DBP and was mainly composed of Pandoraea sp. and Microbacterium sp. From the initial concentrations of 35-500 mg L-1, DBP was efficiently degraded by the consortium, with the degradation rates above 92% within 3 days. The optimal temperature for DBP degradation was 30 degrees C and consortium B1 could adapt to a wide range of pH (5.5-8.5). The analysis of Illumina sequencing further showed that the relative abundance of Pandoraea was increased at the beginning of the degradation, while Microbacterium was decreased. In the later stage of the degradation, the change of the relative abundance of Pandoraea and Microbacterium was opposite. Apart from DBP, consortium B1 could also utilize dimethyl phthalate (DMP), di-2-ethylhexyl phthalate (DEHP), and phthalic acid (PA) as the sole carbon. Moreover, adding B1 to DBP-contaminated soil could greatly improve the removal rate of DBP, suggesting that B1 has a great potential for the bioremediation of DBP-contaminated environments.