Instability of miRNA and cDNAs derivatives in RNA preparations
Instability of miRNA and cDNAs derivatives in RNA preparations
复制标题
DOI：
10.1016/j.bbrc.2006.12.135
复制
发表时间：
2007-02-23
影响因子：
3.1
通讯作者：
Pastori, Ricardo L.
中科院分区：
文献类型：
--
作者：
Bravo, Valia;Rosero, Samuel;Pastori, Ricardo L.
微小RNA（microRNAs，miRNAs）是一类小分子RNA，是基因表达的重要调控因子。我们发现，通常用于miRNA表达研究的RNA制备方法产生高度不稳定的miRNA。我们研究了属于不同miRNAs家族的四种miRNAs的稳定性。在RNA分离后三天，可以观察到这些分子的显著降解。此外，各cDNA也是高度不稳定的。我们的研究结果表明，在设计miRNA表达研究时，应考虑miRNA及其cDNA的不稳定性。(c)2007年由Elsevier Inc.出版
Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies. (c) 2007 Published by Elsevier Inc.