Translational capacity of sheep oocytes microinjected with messenger RNA.
Translational capacity of sheep oocytes microinjected with messenger RNA.
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DOI：
10.1530/jrf.0.0860289
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发表时间：
1989-05
期刊：
影响因子：
--
通讯作者：
R. Moor;D. Powell
中科院分区：
文献类型：
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作者：
R. Moor;D. Powell
绵羊卵母细胞显微注射烟草花叶病毒RNA（TMV-RNA）和同位素标记的L-[35 S]蛋氨酸。总掺入标记的甲硫氨酸是相似的TMV-RNA注射和载体注射的对照卵母细胞，无论是在高蛋白质合成成熟期间或在减少合成的时间相当于中期分裂过渡（48小时后，生发囊泡破裂）期间进行注射。从2.5到10 pg注射的TMV-RNA的量变化对氨基酸掺入的总体水平几乎没有影响。此外，TMV-RNA似乎是非常稳定的卵母细胞和鸡蛋的总多肽合成的比例由TMV-RNA没有减少注射后的第一个48小时。大多数内源性蛋白质的合成均匀减少，以补偿TMV多肽的合成。因此，我们的研究结果表明，绵羊卵母细胞的翻译能力在成熟过程中是完全饱和的。
Sheep oocytes were microinjected with tobacco mosaic virus RNA (TMV-RNA) and isotopically labelled with L-[35S]methionine. Total incorporation of labelled methionine was similar in TMV-RNA-injected and in carrier-injected control oocytes, whether injections were performed during the period of high protein synthesis at maturation or during the period of reduced synthesis at a time equivalent to the mid-cleavage transition (48 h after germinal vesicle breakdown). Varying the amount of TMV-RNA injected from 2.5 to 10 pg had little effect on the overall level of amino acid incorporation. Furthermore TMV-RNA appeared to be very stable in oocytes and eggs; the proportion of total polypeptide synthesis directed by TMV-RNA did not diminish during the first 48 h after injection. Synthesis of most endogenous proteins was uniformly reduced to compensate for the synthesis of TMV-polypeptides. Our results suggest, therefore, that the translational capacity of sheep oocytes is fully saturated during maturation.