Induction of differentiation of the human histocytic lymphoma cell line U-937 by hypericin
Induction of differentiation of the human histocytic lymphoma cell line U-937 by hypericin
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DOI：
10.1007/bf03216751
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发表时间：
1998-02-01
影响因子：
6.7
通讯作者：
Lee, KT
中科院分区：
文献类型：
--
作者：
Kim, JI;Park, JH;Lee, KT
金丝桃素是一种光敏性植物色素，对人髓系白血病U-937细胞有较强的诱导分化作用。在0.2 mU M浓度下，金丝桃素的生长抑制率为50%。金丝桃素对细胞分化的影响是通过其诱导胶乳颗粒的吞噬和还原硝基蓝四氮唑(NET)的能力来评估的。在0.2亩金丝桃素处理的细胞中，约51%的细胞被NET染色，63%的细胞显示吞噬活性。为了确定金丝桃素是否诱导U-937细胞向巨噬细胞或粒细胞分化，测定了酯酶活性和细胞大小。0.2 mU M和0.15 mU M的金丝桃素处理U-937细胞后，α-乙酸萘酯酶活力分别提高了38.4%和48.1%，而萘酚AS-D氯乙酸酯酶活力不受影响。通过流式细胞仪检测，金丝桃素处理的细胞质量的大小比未处理的细胞大。蛋白激酶C(PKC)抑制剂NA-382可降低金丝桃素的净还原活性，而cAMP依赖性蛋白激酶A(PKA)抑制剂H-对分化无影响。这些结果表明，金丝桃素通过刺激PKC诱导向单核巨噬细胞分化。
Hypericin, a photosensitizing plant pigment, was found to be a potent inducer of differentiation of human myeloid leukemia U-937 cells. At a concentration of 0.2 mu M, hypericin exhibited 50% growth inhibition. An effect on cell differentiation by hypericin was assessed by its ability to induce phagocytosis of latex particles, and to reduce nitroblue tetrazolium (NET). Approximately 51% of 0.2 mu M hypericin-treated cells were stained with NET and 63% showed phagocytic activity. In order to establish whether hypericin induces differentiation of U-937 cells to macrophage or granulocyte, esterase activities and cell sizes were measured. When U-937 cells were treated with 0.2 mu M and 0.15 mu M Of hypericin, the alpha-naphthyl acetate esterase activity was increased by 38.4% and 48.1%, respectively, but naphthol AS-D chloroacetate esterase activity was not influenced. The size of hypericin-treated cells in terms of cell mass was larger than that observed in untreated cells as determined by flow cytometry. Protein kinase C (PKC) inhibitor, NA-382, decreased the NET reducing activity of hypericin, whereas a cAMP-dependent protein kinase A (PKA) inhibitor, H-89, did not show any influence on the differentiation. These results indicate that hypericin triggers differentiation toward monocyte:macrophage lineage by PKC stimulation.