Cerebrospinal fluid and serum vascular endothelial growth factor and nitric oxide levels in newborns with hypoxic ischemic encephalopathy
Cerebrospinal fluid and serum vascular endothelial growth factor and nitric oxide levels in newborns with hypoxic ischemic encephalopathy
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DOI：
10.1016/s0387-7604(03)00166-9
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发表时间：
2004-08-01
影响因子：
1.7
通讯作者：
Atalay, Y
中科院分区：
文献类型：
--
作者：
Ergenekon, E;Gücüyener, K;Atalay, Y
兴奋性氨基酸、细胞因子和一氧化氮（NO）在新生儿缺氧缺血性脑病（HIE）的病因和发病机制中起着重要作用。血管内皮生长因子（VEGF）是一种已知的血管生成介质，并已被证明在缺氧期间通过NO介导的机制诱导血管增殖和通透性。本研究旨在探讨新生儿缺氧缺血性脑病（HIE）不同阶段脑脊液和血清中VEGF和NO水平的变化及其相关性。收集19例HIE新生儿和13例对照新生儿出生后24小时内的脑脊液和血清样本，并在-70 ℃下保存直至测量。采用Sievers NOA化学发光法测定NO水平，采用酶联免疫吸附试验双夹心法测定VEGF水平。中、重度HIE患儿脑脊液中NO水平高于对照组和轻度HIE组，轻度HIE患儿脑脊液中VEGF水平高于对照组，中、重度HIE患儿脑脊液中VEGF水平与轻度HIE和对照组相似。两组间血清NO或VEGF水平无差异，CSF和血清中NO和VEGF水平无相关性。根据缺氧损伤的严重程度，VEGF对NO产生的刺激可能对内皮细胞产生不同的影响，这可能导致目前的结果。(C)2003 Elsevier B. V.保留所有权利。
Excitatory amino acids, cytokines and nitric oxide (NO) have been studied in the etiology and pathogenesis of hypoxic ischemic encephalopathy (HIE) of the newborn. Vascular endothelial growth factor (VEGF) is a known mediator of angiogenesis and has been shown to induce vascular proliferation and permeability via NO-mediated mechanism during hypoxia. The objective of this study was to investigate the cerebrospinal fluid and serum VEGF and NO levels in different stages of HIE and the correlation between the two mediators. Cerebrospinal fluid (CSF) and serum samples of 19 newborns with HIE and 13 controls were obtained within the first 24 h of life and kept at -70 degreesC until the time of measurement. NO levels were determined by Sievers NOA by chemiluminescence method and VEGF levels were measured by the enzyme-linked immunosorbent assay double sandwich method. The NO levels in CSF were higher than the control and mild HIE Group in newborns with moderate to severe HIE, and VEGF levels in CSF were higher in the mild HIE group compared to controls but similar in the moderate to severe HIE group compared to mild HIE and control patients. There was no difference between groups with regard to serum NO or VEGF levels, and no correlation was observed between NO and VEGF levels both in CSF and serum samples. Depending on the severity of the hypoxic insult the stimulus for NO production by VEGF may have variable effects on endothelial cells which may give rise to the current results. (C) 2003 Elsevier B.V. All rights reserved.