An EAV-HP insertion in the 5' flanking region of SLCO1B3 is associated with its tissue-expression profile in blue-eggshell Yimeng chickens (Gallus gallus).
An EAV-HP insertion in the 5' flanking region of SLCO1B3 is associated with its tissue-expression profile in blue-eggshell Yimeng chickens (Gallus gallus).
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DOI：
10.1016/j.psj.2020.09.002
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发表时间：
2020-12
期刊：
影响因子：
4.4
通讯作者：
Wu C
中科院分区：
文献类型：
--
作者：
Chen J;Dalirsefat SB;Han D;Dong X;Hua G;Zheng X;Xia T;Shao T;Deng X;Wu C
我们之前报道过，鸡的蓝色蛋壳颜色与溶质载体有机阴离子转运体家族成员1B3 （SLCO1B3）基因启动子区域的部分内源性逆转录病毒（EAV-HP）插入有关。EAV-HP序列包含许多调控元件，这些元件可以调节邻近基因的表达。为了确定这种插入是否会影响邻近基因的表达，我们分别在13只雌性和10只雄性沂蒙鸡的组织中筛选了溶质载体有机阴离子转运蛋白家族成员1C1、1B1 （SLCO1C1、SLCO1B1）和SLCO1B3的表达。我们观察到，插入只显著调节了SLCO1B3的表达，而对SLCO1C1和SLCO1B1的表达没有主要影响。雌性蓝蛋壳鸡的壳腺、大网膜、峡部和输卵管阴道中均检测到SLCO1B3的高表达。我们还在雄性鸡的睾丸中观察到SLCO1B3的异位表达。SLCO1B3通常在肝脏中高表达；然而，EAV-HP的插入显著降低了SLCO1B3的表达。作为肝脏特异性转运体，SLCO1B3表达的降低可能影响肝脏代谢，特别是胆汁酸的代谢。我们还发现，与纯合子基因型相比，EAV-HP插入杂合子基因型的鸟类肺中SLCO1B3的异位表达更高。综上所述，我们证实了EAV-HP插入修饰了SLCO1B3的表达，并首次发现该基因在雌性输卵管和雄性睾丸的所有部位表达谱相似。我们还观察到肝脏中不同水平的SLCO1B3表达，这与EAV-HP插入有关，并且在杂合基因型鸟类的肺部中表达明显更高。SLCO1B3表达模式的这些变化对组织功能的影响值得进一步研究。
We previously reported that blue eggshell color in chickens is associated with a partial endogenous retroviral (EAV-HP) insertion in the promoter region of the solute carrier organic anion transporter family member 1B3 (SLCO1B3) gene. The EAV-HP sequence includes numerous regulatory elements, which may modulate the expression of adjacent genes. To determine whether this insertion influences the expression of neighboring genes, we screened the expression of solute carrier organic anion transporter family members 1C1, 1B1 (SLCO1C1, SLCO1B1), and SLCO1B3 in 13 and 10 tissues from female and male Yimeng chickens, respectively. We observed that the insertion only significantly modulated the expression of SLCO1B3 and did not majorly affect that of SLCO1C1 and SLCO1B1. High expression of SLCO1B3 was detected in the shell gland, magnum, isthmus, and vagina of the oviduct in female blue-eggshell chickens. We also observed ectopic expression of SLCO1B3 in the testes of male chickens. SLCO1B3 is typically highly expressed in the liver; however, the EAV-HP insertion significantly reduces SLCO1B3 expression. As a liver-specific transporter, a reduction in the expression of SLCO1B3 may affect liver metabolism, particularly that of bile acids. We also detected higher ectopic expression of SLCO1B3 in the lungs of birds heterozygous for the EAV-HP insertion than in homozygous genotypes. In conclusion, we confirmed that the EAV-HP insertion modifies SLCO1B3 expression, and showed, for the first time, similar expression profile of this gene in all parts of the oviduct in females and testis in males. We also observed different levels of SLCO1B3 expression in the liver, which were associated with the EAV-HP insertion, and significantly higher expression in the lungs of birds with heterozygous genotype. The effects of these changes in the SLCO1B3 expression pattern on the function of the tissues warrant further investigation.
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影响因子：
--
作者：
Pizzagalli, F;Hagenbuch, B;Meier, PJ
通讯作者：
Meier, PJ
影响因子：
29.4
作者：
Ismair, MG;Stieger, B;Kullak-Ublick, GA
通讯作者：
Kullak-Ublick, GA
DOI：
10.1016/0305-0491(76)90183-8
发表时间：
1976-01-01
影响因子：
2.2
作者：
KENNEDY, GY;VEVERS, HG
通讯作者：
VEVERS, HG
影响因子：
6.3
作者：
Ohtsuka, H;Abe, T;Unno, M
通讯作者：
Unno, M
影响因子：
5.4
作者：
Sacco, MA;Flannery, DMJ;Venugopal, K
通讯作者：
Venugopal, K