Morphogenesis of Avian Infectious Bronchitis Virus and a Related Human Virus (Strain 229E)
Morphogenesis of Avian Infectious Bronchitis Virus and a Related Human Virus (Strain 229E)
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DOI：
10.1128/jvi.1.5.1019-1027.1967
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发表时间：
1967-10
影响因子：
5.4
通讯作者：
W. Becker;K. Mcintosh;J. Dees;R. Chanock
中科院分区：
文献类型：
--
作者：
W. Becker;K. Mcintosh;J. Dees;R. Chanock
鸡传染性支气管炎病毒（IBV）和229 E株（最近从感冒患者中回收的一种病毒）在负染色制备物中显示具有相似的独特形态。本文用电镜观察了鸡传染性支气管炎病毒（IBV）在鸡胚绒毛尿囊膜中的形态发生和229 E株在WI-38细胞中的形态发生。在感染的细胞中，观察到直径82 mμ的圆形电子致密颗粒通过从内质网膜和胞质小泡的出芽过程形成。IBV感染细胞中的颗粒与229 E株感染细胞中的颗粒大小和形状相似，但内部结构存在一定差异。粒子代表病毒粒子的证据和这些发现在这个病毒组的分类的影响进行了讨论。
Avian infectious bronchitis virus (IBV) and strain 229E, a virus recently recovered from patients with colds, have been shown to possess a similar distinctive morphology in negatively stained preparations. An electron microscopic study of the morphogenesis of IBV in the chorioallantoic membrane and of strain 229E in WI-38 cells was performed. In infected cells, round electron-dense particles 82 mμ in diameter were observed to form by a process of budding from membranes of the endoplasmic reticulum and cytoplasmic vesicles. The particles in IBV-infected cells were similar in size and shape to those in strain 229E-infected cells but showed certain differences in internal structure. The evidence that the particles represent virions and the implications of these findings in the classification of this virus group are discussed.