Effect of verapamil and sulfhydryl reagents on calcium transport in bovine spermatozoa.
Effect of verapamil and sulfhydryl reagents on calcium transport in bovine spermatozoa.
复制标题
维拉帕米和巯基试剂对牛精子钙转运的影响。
DOI：
10.1095/biolreprod36.3.658
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发表时间：
1987
影响因子：
3.6
通讯作者：
Lardy,HA
中科院分区：
文献类型：
--
作者：
Breitbart,H;Lardy,HA
完整牛附睾精子对钙的吸收不受低浓度（高达 0.75 mM）钙转运阻滞剂维拉帕米的影响。在这些条件下，钙转运到精子线粒体中受到高度抑制。在较高的维拉帕米浓度（1.0、1.5 mM）下，钙转运到完整精子中也会受到抑制，并且这种抑制不能通过用菲律宾素破坏质膜来缓解。完整精子对钙的吸收受到 mersalyl 的高度抑制，当质膜被 filipin 破坏时，这种抑制作用可以完全缓解。 mersalyl 的这种作用并不依赖于培养介质中磷酸盐的存在。磷酸盐本身高达 2 mM，可增强细胞对钙的吸收；这种效应在浓度较高时减弱，在 10 mM 磷酸盐时抑制 57%。高磷酸盐浓度的这种抑制作用可以被 mersalyl 阻断。这表明钙载体本身而不是质膜的磷酸盐载体被mersalyl抑制。牛精子的质膜中可能存在钙和磷酸盐的同向转运蛋白。
Calcium uptake by intact bovine epididymal spermatozoa is not affected by low concentrations (up to 0.75 mM) of the calcium transport blocker verapamil. Under these conditions, calcium transport into sperm mitochondria is highly inhibited. At higher verapamil concentrations (1.0, 1.5 mM), calcium transport into intact sperm is also inhibited, and this inhibition cannot be relieved by disrupting the plasma membrane with filipin. Calcium uptake into intact sperm is highly inhibited by mersalyl and this inhibitory effect can be completely relieved when the plasma membrane is disrupted by filipin. This effect of mersalyl is not dependent on the presence of phosphate in the incubation medium. Phosphate itself, up to 2 mM, enhances calcium uptake into the cells; this effect decreases at higher concentrations and is depressed 57% at 10 mM phosphate. This inhibitory effect of high phosphate concentration can be blocked by mersalyl. It is suggested that the calcium carrier itself and not a phosphate carrier of the plasma membrane is inhibited by mersalyl. It is possible that there is a symporter for calcium and phosphate in the plasma membrane of bovine spermatozoa.