Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family
Molecular properties of Zic4 and Zic5 proteins: functional diversity within Zic family
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DOI：
10.1016/j.bbrc.2004.09.052
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发表时间：
2004-11-05
影响因子：
3.1
通讯作者：
Aruga, J
中科院分区：
文献类型：
--
作者：
Ishiguro, A;Inoue, T;Aruga, J
zic家族蛋白控制着多种发育过程。已有研究表明，Zic1、Zic2和Zic3可作为转录调控因子，其功能受到I-mfa的抑制，而I-mfa已被鉴定为基本螺旋-环-螺旋型转录因子的抑制因子。在这里，我们研究了Zic4和Zic5蛋白的分子特性。与Zic1/Zic2/Zic3蛋白类似，Zic4/Zic5蛋白对gli结合序列具有dna结合活性。然而，Zic4/Zic5与Zic1/Zic2/Zic3结合I-mfa的能力并无显著差异。Zic4/Zic5的核定位不受I-mfa蛋白存在的影响，而Zic1/Zic2/Zic3蛋白在I-mfa存在的情况下转移到细胞质室。这种差异可能是由于Zic1/Zic2/Zic3和Zic4/Zic5蛋白之间的n端区域不同，因为Zic1/Zic2/Zic3蛋白与I-mfa的结合是通过它们的n端区域发生的。(C) 2004爱思唯尔公司版权所有。
The Zic-family proteins control various developmental processes. Previous studies have shown that Zic1, Zic2, and Zic3 can act as transcriptional regulators, and that their functions are repressed by I-mfa, which has been identified as a repressor for basic helix-loop-helix-type transcriptional factors. Here, we investigated the molecular properties of the Zic4 and Zic5 proteins. Zic4/Zic5 showed DNA-binding activity to the Gli-binding sequence, similar to Zic1/Zic2/Zic3 proteins. However, Zic4/Zic5 did not exhibit any significant transcriptional activation ability nor they bind to I-mfa differently from Zic1/Zic2/Zic3. The nuclear localization of Zic4/Zic5 was not affected by the presence of the I-mfa protein, whereas the Zic1/Zic2/Zic3 proteins were translocated to the cytoplasmic compartment in the presence of I-mfa. The difference may be attributable to the dissimilarity of the N-terminal region between the Zic1/Zic2/Zic3 and Zic4/Zic5 proteins, since the binding of the Zic1/Zic2/Zic3 proteins to I-mfa occurs through their N-terminal regions. (C) 2004 Elsevier Inc. All rights reserved.