Quantification of binary diffusion in protein crystals
Quantification of binary diffusion in protein crystals
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DOI：
10.1021/jp050289c
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发表时间：
2005-06-02
影响因子：
3.3
通讯作者：
van der Wielen, LAM
中科院分区：
文献类型：
--
作者：
Cvetkovic, A;Picioreanu, C;van der Wielen, LAM
使用共聚焦激光扫描显微镜的可视化和量化的二元扩散内各向异性多孔材料在这里首次描述。随后分析了双阴离子荧光素、两性离子罗丹明B及其混合物在阳离子天然正交晶系溶菌酶晶体中的吸附动力学。所有数据可用经典的孔扩散模型描述。有没有变化的吸附特性，但扩散减少与引入第二溶质在溶液中。结果发现，扩散是由空间和静电相互作用的组合，而吸附是依赖于静电和疏水相互作用。因此，可以确定二元输运的结果取决于溶质、蛋白质和晶体特征。
The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics.