FREQUENCY OF T-CELLS SPECIFIC FOR MYELIN BASIC-PROTEIN AND MYELIN PROTEOLIPID PROTEIN IN BLOOD AND CEREBROSPINAL-FLUID IN MULTIPLE-SCLEROSIS
FREQUENCY OF T-CELLS SPECIFIC FOR MYELIN BASIC-PROTEIN AND MYELIN PROTEOLIPID PROTEIN IN BLOOD AND CEREBROSPINAL-FLUID IN MULTIPLE-SCLEROSIS
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DOI：
10.1016/0165-5728(92)90095-3
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发表时间：
1992-05-01
影响因子：
3.3
通讯作者：
VANDENBARK, AA
中科院分区：
文献类型：
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作者：
CHOU, YK;BOURDETTE, DN;VANDENBARK, AA
T细胞对髓鞘碱性蛋白(MBP)和髓鞘蛋白脂蛋白(PLP)两种髓鞘成分的敏感性可能在多发性硬化(MS)的发病机制中起重要作用。采用极限稀释法，我们证明MS患者的血液中MBP反应性T细胞的频率高于正常人、其他神经系统疾病(OND)和类风湿关节炎患者。合成肽PLP139-151或单纯疱疹病毒的T细胞频率没有差异。在脑脊液中，37%的MS患者的IL-2/IL-4反应性T细胞分离株对MBP或PLP139-151有反应，而OND患者的类似分离株只有5%对这些髓鞘抗原有反应。MS患者脑脊液中MBP反应性T细胞的平均相对频率明显高于OND患者(22×10~(-5)细胞和1×10~(-5)细胞)，与实验性自身免疫性脑脊髓炎大鼠中枢神经系统的MBP反应性T细胞相似。这些结果为髓鞘反应性T细胞介导MS疾病的假设提供了新的支持。
T cell sensitization to two myelin components, myelin basic protein (MBP) and myelin proteolipid protein (PLP), may be important to the pathogenesis of multiple sclerosis (MS). Using the limiting dilution assay, we demonstrated that the blood of MS patients had an increased frequency of MBP-reactive T cells compared with normal subjects and patients with other neurological diseases (OND) and rheumatoid arthritis. There was no difference in T cell frequency to a synthetic peptide, PLP139-151, or Herpes simplex virus. Within cerebrospinal fluid (CSF), 37% of IL-2/IL-4-reactive T cell isolates from MS patients responded either to MBP or PLP139-151 while only 5% of similar isolates from OND patients responded to these myelin antigens. The mean relative frequency of MBP-reactive T cells within CSF from MS patients was significantly higher than that of OND patients (22 x 10(-5) cells versus 1 x 10(-5) cells) and was similar to that of MBP reactive T cells within the central nervous system of rats with experimental autoimmune encephalomyelitis. These results lend new support to the hypothesis that myelin-reactive T cells mediate disease in MS.