Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress
Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress
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DOI：
10.1016/j.jep.2007.01.004
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发表时间：
2007-05-22
影响因子：
5.4
通讯作者：
Lee, Chang Y.
中科院分区：
文献类型：
--
作者：
Kim, Kyung Tack;Yoo, Kyung Mi;Lee, Chang Y.
热加工的亚洲人参根（Panax ginseng C.A. Meyer），在亚洲也被称为“红参”，据报道比未经加工的白色人参根具有更高的生物活性。因此，西洋参（Panax quinquefolius L.）对红参进行了热处理，并检测了加热过程中生物活性的变化。将西洋参在100 ℃下蒸煮30、60、90和120 min，通过测定西洋参皂苷和总酚含量、DPPH自由基和超氧阴离子自由基清除能力、对V79-4细胞活力和脂质过氧化的保护作用，研究西洋参的生物活性。热处理使人参总皂苷含量（4.97%，w/w）比白色人参（3.27%，w/w）和总酚含量从444.5 mg GAE/100 g增加到489.6-574.2 mg GAE/100 g。抗氧化活性也从285 mg/100 g（维生素C当量）增加到353-487 mg/100 g。热处理人参对DPPH自由基的清除率为59.5-88.5%，对超氧阴离子自由基的清除率为44.2-90.9%。加热的人参保护细胞活力免受H2 O2诱导的氧化损伤，并增强超氧化物歧化酶和过氧化氢酶的活性在V79-4细胞中呈剂量依赖性。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Heat-processed Asian ginseng roots (Panax ginseng C.A. Meyer), also known as "red ginseng" in Asia, are reported to have more bioactivity than the no-processed white ginseng roots. Therefore, American fresh ginseng roots (Panax quinquefolius L.) were processed to the red ginseng and examined changes in bioactivity during heating process. The fresh America ginseng roots were steamed at 100 degrees C for 30, 60, 90 and 120 min, and their bioactivities were examined by analyzing the content of ginsenosides and total phenolics, and measuring DPPH and superoxide radical scavenging acivity and their protective effects on V79-4 cells viability and lipid peroxidation. The heating treatment proportionally increased total ginsenosides (4.97%, w/w) content compared with white ginseng (3.27%) and total phenolics from 444.5 mg GAE/100 g to 489.6-574.2 mg GAE/100 g. The antioxidant activity also increased from 285 mg/100 g (vitamin C equivalent) to 353-487 mg/100 g. Heated ginseng showed high levels of DPPH radical scavenging activity (59.5-88.5%) and the high level of superoxide radical scavenging activity (44.2-90.9%). The heated ginseng protected cell viability against H2O2-induced oxidative damage, and enhanced the activities of superoxide dismutase and catalase by dose dependently in V79-4 cells. (c) 2007 Elsevier Ireland Ltd. All rights reserved.