TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-β1 and IL-10 and tumor cells migration
TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-β1 and IL-10 and tumor cells migration
复制标题
DOI：
10.3233/bme-130879
复制
发表时间：
2014-01-01
影响因子：
1
通讯作者：
Gai, Xiaodong
中科院分区：
文献类型：
--
作者：
Li, Chun;Li, Haixia;Gai, Xiaodong
触发肿瘤细胞逃避免疫监视的信号通路尚不完全清楚。toll样受体（TLRs）被认为在免疫细胞和肿瘤细胞中均表达。通过检测小鼠Lewis肺癌（LLC）细胞与小鼠淋巴细胞共培养前后TLRs的表达，得出LLC细胞组成性表达TLR1、TLR2、TLR3、TLR4、TLR5、TLR6和TLR9。同时，TLR4在LLC细胞与小鼠淋巴细胞共培养后表达最强。为了探讨TLR4信号通路在LLC中的可能作用，采用ELISA法检测LLC细胞上清中tgf - β 1和IL-10蛋白的浓度，并采用transwell法检测LPS刺激LLC细胞后的迁移情况。FCM法检测LPS刺激后LLC细胞中TLR4蛋白的表达。结果表明，LPS刺激后tgf - β 1和IL-10蛋白水平均显著升高，并在LPS作用24 h和10 μ g/mL时达到最大值。LPS刺激下的LLC细胞迁移量显著增加，在LPS浓度为10 μ g/mL时达到最大值。LPS刺激10 μ g/mL后，TLR4蛋白表达显著增强。这些结果提示，肺癌细胞中TLR4信号通路的激活可能通过促进TGF-mu 1和IL-10的表达以及肿瘤细胞的迁移参与肿瘤的逃逸和进展。
The signaling pathways that trigger tumor cell escape from immune surveillance are not understood completely. Toll-like receptors (TLRs) are considered to be expressed in both immune cells and tumor cells. By detecting TLRs expression in mouse Lewis lung cancer (LLC) before and after co-culture with mouse lymphocytes, the authors concluded that LLC cells constitutively expressed TLR1, TLR2, TLR3, TLR4, TLR5, TLR6 and TLR9. Meanwhile, TLR4 expression in LLC cells was the strongest after co-culture with mouse lymphocytes. To investigate the possible roles of TLR4 signaling pathway in LLC, the concentrations of TGF-beta 1 and IL-10 protein in LLC cells supernatant were detected by ELISA, and the migration of LLC cells were detected by transwell assay after lipopolysaccharide (LPS) stimulation. TLR4 protein expression in LLC cells was also detected after LPS stimulation by FCM. The results indicated that both levels of TGF-beta 1 and IL-10 protein were significantly increased after LPS stimulation and reached to a maximum at 24 h and 10 mu g/mL of LPS. The migrated LLC cells with LPS stimulation were significantly increased and reached to a maximum at 10 mu g/mL of LPS. The expression of TLR4 protein was significantly enhanced after 10 mu g/mL of LPS stimulation. These results suggest that the activation of TLR4 signaling pathway in lung cancer cells may be involved in tumor escape and progression by promoting the expression of TGF-mu 1 and IL-10 and tumor cells migration.