Characterization of an Anisotropic Hydrogel Tissue Substrate for Infusion Testing.
Characterization of an Anisotropic Hydrogel Tissue Substrate for Infusion Testing.
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用于输注测试的各向异性水凝胶组织基质的表征。
DOI：
10.1002/app.30639
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Sarntinoranont,Malisa
中科院分区：
文献类型：
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作者：
Lee,SungJin;Pishko,GregoryL;Astary,GarretW;Mareci,ThomasH;Sarntinoranont,Malisa
捕获特定运输特性的人工组织模型对于研究对药物输送重要的物理现象是有用的。在本研究中，以模拟排列组织为目标，建立了一种蛋白体外组织模型并进行了表征。通过构建1%琼脂糖水凝胶，植入不同体积分数（~ 5、10和20%）直径为10 μm的玻璃纤维，形成了各向异性多孔介质。在直接输注大分子示踪剂Evans蓝白蛋白（EBA）后，开发的底物能够捕获各向异性运输。为了进一步表征测试基板，通过扩散张量成像测量了水的扩散张量，在纤维体积分数为5%、10%和20%的情况下，水在与玻璃纤维平行和垂直方向上的扩散系数分别为1.16、1.20和1.26。在与植入纤维平行的可控微流条件下，通过测量样品间的压力梯度来估计水力导电性。测定了不同浓度无纤维水凝胶和1%含不同纤维体积分数水凝胶的水导率；例如，当纤维体积分数为20%时，K∥= 1.20 × 10−12m4N−1s−1（其中，K∥为平行于玻璃纤维方向的电导率分量）。此外，EBA分布拟合多孔介质输运模型，以估计垂直于玻璃纤维方向的水力导电性。在纤维体积分数为5%、10%和20%的情况下，估计的定向水力导电性比值K∥/K⟂（其中，⟂为垂直于玻璃纤维方向的导电性分量）的范围分别约为3 ~ 5、6 ~ 10和40 ~ 90。这些琼脂糖水凝胶模型为定量低流速输注方案提供了方便的介质。©2009 Wiley期刊公司高分子学报，2009
Artificial tissue models that capture specific transport properties are useful for investigating physical phenomena important to drug delivery. In this study, anin vitrotissue model was developed and characterized with the goal of mimicking aligned tissue. An anisotropic porous medium was developed by the construction of a 1% agarose hydrogel implanted with different volume fractions (∼ 5, 10, and 20%) of 10‐μm‐diameter glass fibers. The developed substrate was able to capture anisotropic transport after the direct infusion of a macromolecular tracer, Evans blue albumin (EBA). To further characterize the test substrate, the diffusion tensor of water was measured by diffusion tensor imaging, and the ratios of the diffusivities in the directions parallel and perpendicular to the glass fibers were 1.16, 1.20, and 1.26 for 5, 10, and 20% fiber volume fractions, respectively. The hydraulic conductivity was estimated by the measurement of pressure gradients across samples under controlled microflow conditions in the direction parallel to implanted fibers. The hydraulic conductivities at various hydrogel concentrations without fibers and in a 1% hydrogel with various fiber volume fractions were measured; for example,K∥= 1.20 × 10−12m4N−1s−1(whereK∥is the conductivity component in the direction parallel to the glass fibers) for 20% fiber volume fractions. Also, EBA distributions were fit to porous medium transport models to estimate hydraulic conductivity in the direction perpendicular to glass fibers. The estimated ratio of directional hydraulic conductivity,K∥/K⟂(whereK⟂is the conductivity component in the direction perpendicular to the glass fibers), ranged from approximately 3 to 5, from 6 to 10, and from 40 to 90 for 5, 10, and 20% fiber volume fractions, respectively. These agarose hydrogel models provided convenient media for quantifying infusion protocols at low flow rates. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009