Hourglass model for a protein-based circadian oscillator
Hourglass model for a protein-based circadian oscillator
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DOI：
10.1103/physrevlett.96.038303
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发表时间：
2006-01-27
影响因子：
8.6
通讯作者：
Wingreen, NS
中科院分区：
文献类型：
--
作者：
Emberly, E;Wingreen, NS
许多生物体拥有内部生化钟，称为昼夜节律振荡器，允许它们以24小时为周期调节其生物活动。最近发现，光合蓝藻的昼夜振荡器能够在试管中仅用三种蛋白质KaiA、KaiB和KaiC以及ATP发挥作用。生化事件本质上是随机的，这往往会使振荡的蛋白质群体去随机化。我们提出，Kai蛋白质振荡器的稳定性依赖于主动同步（i）白天KaiC六聚体之间的单体交换，以及（ii）夜间KaiC六聚体簇的形成。我们的研究结果强调了生物化学网络中蛋白质的集体组装或拆卸的重要性，并可能有助于指导新型蛋白质振荡器的设计。
Many organisms possess internal biochemical clocks, known as circadian oscillators, which allow them to regulate their biological activity with a 24-hour period. It was recently discovered that the circadian oscillator of photosynthetic cyanobacteria is able to function in a test tube with only three proteins, KaiA, KaiB, and KaiC, and ATP. Biochemical events are intrinsically stochastic, and this tends to desynchronize oscillating protein populations. We propose that stability of the Kai-protein oscillator relies on active synchronization by (i) monomer exchange between KaiC hexamers during the day, and (ii) formation of clusters of KaiC hexamers at night. Our results highlight the importance of collective assembly or disassembly of proteins in biochemical networks, and may help guide design of novel protein-based oscillators.