The origin of in situ hybridization - A personal history.
The origin of in situ hybridization - A personal history.
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DOI：
10.1016/j.ymeth.2015.11.026
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发表时间：
2016-04-01
期刊：
影响因子：
--
通讯作者：
Gall JG
中科院分区：
文献类型：
--
作者：
Gall JG
原位杂交是在细胞学制备物中检测特定RNA或DNA分子的技术。基本上，它涉及在细胞中的内源性单链RNA或DNA与互补单链RNA或DNA探针之间形成杂交分子。在其原始形式中，探针用3H标记，并通过放射自显影法检测杂交体。1968年的第一次成功实验涉及检测蛙爪蟾卵母细胞中高度扩增的核糖体DNA，随后不久在小鼠和果蝇染色体中重复了“卫星DNA”。大约十年后，荧光探针被开发出来。
In situ hybridization is the technique by which specific RNA or DNA molecules are detected in cytological preparations. Basically it involves formation of a hybrid molecule between an endogenous single-stranded RNA or DNA in the cell and a complementary single-stranded RNA or DNA probe. In its original form the probe was labeled with 3H and the hybrid was detected by autoradiography. The first successful experiments in 1968 involved detection of the highly amplified ribosomal DNA in oocytes of the frog Xenopus, followed soon after by the reiterated “satellite DNA” in mouse and Drosophila chromosomes. Fluorescent probes were developed about ten years later.