Circulating rotavirus-specific antibody-secreting cells (ASCs) predict the presence of rotavirus-specific ASCs in the human small intestinal lamina propria
Circulating rotavirus-specific antibody-secreting cells (ASCs) predict the presence of rotavirus-specific ASCs in the human small intestinal lamina propria
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DOI：
10.1086/315808
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发表时间：
2000-10-01
影响因子：
6.4
通讯作者：
Offit, PA
中科院分区：
文献类型：
--
作者：
Brown, KA;Kriss, JA;Offit, PA
轮状病毒是全世界儿童感染性腹泻的最重要原因。保护作用很可能是由小肠病毒特异性伊加介导的。然而，无论是粪便或血清病毒特异性伊加明确与保护免受挑战。评估了循环中轮状病毒特异性抗体和轮状病毒特异性抗体分泌细胞（ASC）预测儿童肠道中ASC存在的能力。从21名儿童的肠道活检样本和血液中的单核细胞富集了CD 38（终末分化B细胞的标志物），并通过ELISPOT测定评估了病毒特异性和总伊加和IgG分泌细胞的存在。血清病毒特异性伊加和IgG水平通过ELISA测定。血液中病毒特异性IgA分泌细胞与总IgA分泌细胞的比例与小肠中的比例相关，但与大肠无关。相反，血清轮状病毒特异性伊加与小肠中病毒特异性ASC的存在相关性较低。
Rotaviruses are the most important cause of infectious diarrhea in children throughout the world. Protection is most likely mediated by small-intestinal virus-specific IgA. However, neither fecal nor serum virus-specific IgA clearly correlates with protection against challenge. The capacity of rotavirus-specific antibodies and rotavirus-specific antibody-secreting cells (ASCs) in the circulation to predict the presence of ASCs in the intestines of children was evaluated. Mononuclear cells from intestinal biopsy samples and blood from 21 children were enriched for CD38, a marker of terminally differentiated B cells, and evaluated for the presence of virus-specific and total IgA- and IgG-secreting cells, by ELISPOT assay. Serum virus-specific IgA and IgG levels were determined by ELISA. The ratio of virus-specific to total IgA-secreting cells in the blood correlated with that found in the small, but not large, intestine. In contrast, serum rotavirus-specific IgA correlated less well with the presence of virus-specific ASCs in the small intestine.