PROTECTION AGAINST MALARIA BY VACCINATION WITH SPOROZOITE SURFACE PROTEIN-2 PLUS CS PROTEIN
PROTECTION AGAINST MALARIA BY VACCINATION WITH SPOROZOITE SURFACE PROTEIN-2 PLUS CS PROTEIN
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DOI：
10.1126/science.1827210
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发表时间：
1991-05-03
期刊：
影响因子：
56.9
通讯作者：
HOFFMAN, SL
中科院分区：
文献类型：
--
作者：
KHUSMITH, S;CHAROENVIT, Y;HOFFMAN, SL
十年来，环孢子子（CS）蛋白一直是开发疟疾孢子子疫苗的目标。然而，以CS蛋白为基础的亚单位疫苗免疫从来没有像辐照孢子虫免疫后那样提供完全的保护。用辐照过的约氏疟原虫孢子子免疫BALB/c小鼠，产生抗体和细胞毒性T细胞，对抗140千顿的孢子子表面蛋白2 （SSP2）。用转染了SSP2或CS基因的P815细胞免疫小鼠可获得部分保护，而用SSP2和CS混合转染的P815细胞免疫小鼠可获得完全保护。这些研究强调了疫苗递送系统在实现保护方面的重要性，并定义了一种多抗原孢子子疫苗。
The circumsporozoite (CS) protein has been the target for development of malaria sporozoite vaccines for a decade. However, immunization with subunit vaccines based on the CS protein has never given the complete protection found after immunization with irradiated sporozoites. BALB/c mice immunized with irradiated Plasmodium yoelii sporozoites produced antibodies and cytotoxic T cells against a 140-kilodalton protein, sporozoite surface protein 2 (SSP2). Mice immunized with P815 cells that had been transfected with either SSP2 or CS genes were partially protected, and those immunized with a mixture of SSP2 and CS transfectants were completely protected against malaria. These studies emphasize the importance of vaccine delivery systems in achieving protection and define a multi-antigen sporozoite vaccine.