Development of a radioimmunoassay for isometamidium
Development of a radioimmunoassay for isometamidium
复制标题
DOI：
10.1007/bf00343258
复制
发表时间：
2004
影响因子：
2.2
通讯作者：
L. Kinabo;J. Bogan
中科院分区：
文献类型：
--
作者：
L. Kinabo;J. Bogan
本文报道了一种测定杀锥虫药异美他啶（ISMM）的放射免疫分析方法。用14 C-ISMM作为放射性配体，测定了抗体的交叉反应性，发现与吡啶啶、亚胺氮和喹吡胺的交叉反应性小于3%，与人啶的交叉反应性约为13%。血清中ISMM的检测限为5.8 pmol/管，批内和批间变异系数分别为4和14%。
A radioimmunoassay for the trypanocidal drug isometamidium (ISMM) has been developed using antibodies produced in sheep immumzed against the drug cross-linked to human serum albumin. With14C-ISMM as a radioligand, cross-reactivity of the antibodies was determined and found to be less than 3% with pyrithidium, diminazene and quinapyramine and about 13% with homidium. The limit of detection of ISMM in serum without prior extraction was 5.8 pmol/tube and within-assay and between-assay coefficients of variation were four and 14% respectively.