A comparison of muscle- and scale-derived delta13C and delta15N across three life-history stages of Atlantic salmon, Salmo salar.
A comparison of muscle- and scale-derived delta13C and delta15N across three life-history stages of Atlantic salmon, Salmo salar.
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DOI：
10.1002/rcm.3674
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
R. Sinnatamby;J. Dempson;M. Power
中科院分区：
文献类型：
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作者：
R. Sinnatamby;J. Dempson;M. Power
从成对的鳞片和肌肉组织样本中获得了稳定的同位素特征，这些样本来自Smolt、Smolt后和一次海冬季成体大西洋鲑鱼(Salmo Salar)。将后期鳞片和成虫鳞片分为中心部分和外部(海洋)部分，对生活史阶段的海洋生长部分和成人鳞片的中心部分进行了分析。肌肉和鳞片增量(13)C和增量(15)N特征被评估(1)以确定组织类型之间是否存在线性关系，(2)确定在所有生命历史阶段的组织特征之间是否存在恒定的偏移量，以及(3)评估垫底是否对生命历史尺度片段造成显著的偏差，从而排除了它们在生命历史阶段之间的任何个体发育饮食变化的回顾分析中的使用。肌肉和鳞片稳定的同位素特征之间存在着显著的相关性，这些特征来自黑斑羚(Delta(13)C和Delta(15)N)和成体(Delta(15)N)。肌肉和鳞片的特征都捕捉到了与从淡水环境向海洋环境过渡相关的饮食变化。成虫和成虫的鳞片相对于肌肉组织是耗尽的，这可能是由于肌肉和鳞片组织之间氨基酸组成的同位素差异所致。结果表明，鳞片可能更好地代表饮食碳源，因为它们不受脂质动态的影响。然而，该量表似乎对饮食相对于肌肉的短期变化不太敏感，因此只能用来推断生长缓慢的生活史阶段的季节性综合饮食模式。
Stable isotope signatures were obtained from paired scale and muscle tissue samples from smolt, post-smolt and one-sea-winter adult Atlantic salmon (Salmo salar). Post-smolt and adult scales were separated into central and outer (marine) portions with analyses carried out on the marine growth section of both life-history stages and the central portion for the adult scales. Muscle and scale delta(13)C and delta(15)N signatures were assessed (1) to determine whether a linear relationship exists between tissue types, (2) to determine if a constant offset exists between tissue signatures across all life-history stages, and (3) to evaluate whether underplating imparts a significant bias to life-history scale segments that would preclude their use in retrospective analyses of any ontogenetic dietary changes between life-history stages. Significant correlations were found to exist between muscle and scale stable isotope signatures obtained from smolts (delta(13)C and delta(15)N) and adults (delta(15)N). Both the muscle and the scale signatures captured the dietary shift associated with the transition from freshwater to the marine environment. Post-smolt and adult scales were depleted relative to muscle tissue, which may be attributed to isotopic differences in amino acid composition between muscle and scale tissues. The results suggest that scales may better represent dietary carbon sources because they are not influenced by lipid dynamics. The scale, however, appears less responsive to short-term shifts in diet relative to muscle and, therefore, must be used only to infer seasonally integrated dietary patterns for slow-growing life-history stages.