Ribosomal Chamber Music: Toward an Understanding of IRES Mechanisms.
Ribosomal Chamber Music: Toward an Understanding of IRES Mechanisms.
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DOI：
10.1016/j.tibs.2017.06.002
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发表时间：
2017-08
影响因子：
13.8
通讯作者：
Hiroshi Yamamoto;A. Unbehaun;C. Spahn
中科院分区：
文献类型：
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作者：
Hiroshi Yamamoto;A. Unbehaun;C. Spahn
内部起始是一种 5' 端独立的翻译起始模式，由许多病毒和推定的一些细胞编码模板参与。内部起始是由特定的 RNA 三级折叠促进的，称为内部核糖体进入位点 (IRES)，位于各个转录本的 5' 非翻译区 (UTR)。在这篇综述中，我们讨论了最新的结构见解，即已建立的 IRES 如何首先捕获然后通过非规范相互作用和引导真核核糖体的内在构象灵活性来操纵真核翻译机制。由于 IRES 的操作复杂性降低，并且构成了最小的起始系统，因此与规范起始进行比较可以描述核糖体的常见机械原理。
Internal initiation is a 5′-end-independent mode of translation initiation engaged by many virus- and putatively some cell-encoded templates. Internal initiation is facilitated by specific RNA tertiary folds, called internal ribosomal entry sites (IRESs), in the 5′ untranslated region (UTR) of the respective transcripts. In this review we discuss recent structural insight into how established IRESs first capture and then manipulate the eukaryotic translation machinery through non-canonical interactions and by guiding the intrinsic conformational flexibility of the eukaryotic ribosome. Because IRESs operate with reduced complexity and constitute minimal systems of initiation, comparison with canonical initiation may allow common mechanistic principles of the ribosome to be delineated.