Marine subsurface eukaryotes: the fungal majority
Marine subsurface eukaryotes: the fungal majority
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DOI：
10.1111/j.1462-2920.2010.02318.x
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发表时间：
2011-01-01
影响因子：
5.1
通讯作者：
Teske, Andreas
中科院分区：
文献类型：
--
作者：
Edgcomb, Virginia P.;Beaudoin, David;Teske, Andreas
P>对深层地下沉积物微生物群落的研究表明，整个沉积物柱中存在细菌和古细菌。微生物真核生物也可能存在于深海地下沉积物中；能够吸收埋藏有机碳的食菌原生生物或真核生物。这里使用基于 DNA 和 RNA 的克隆文库分析来检查微生物真核多样性，并识别秘鲁边缘和秘鲁海沟深海沉积物核心中的潜在活性成员。我们将表面群落与同一核心中更深层的群落进行了比较，并比较了来自不同地点的核心。真菌序列最常从基于 DNA 和 RNA 的克隆文库中回收，在基于 RNA 和基于 DNA 的文库中，不同序列类型和不同显性克隆类型的总体丰度存在差异。表面沉积物群落彼此不同，也与深层地下样本不同。一些真菌序列代表了潜在的新生物体以及在陆地、淡水和咸水环境中广泛分布的生物体。我们的结果表明，真菌是海洋沉积物地下最一致检测到的真核生物；此外，一些物种可能特别适应地下深处，并可能在养分的利用和循环中发挥重要作用。
P>Studies on the microbial communities of deep subsurface sediments have indicated the presence of Bacteria and Archaea throughout the sediment column. Microbial eukaryotes could also be present in deep-sea subsurface sediments; either bacterivorous protists or eukaryotes capable of assimilating buried organic carbon. DNA- and RNA-based clone library analyses are used here to examine the microbial eukaryotic diversity and identify the potentially active members in deep-sea sediment cores of the Peru Margin and the Peru Trench. We compared surface communities with those much deeper in the same cores, and compared cores from different sites. Fungal sequences were most often recovered from both DNA- and RNA-based clone libraries, with variable overall abundances of different sequence types and different dominant clone types in the RNA-based and the DNA-based libraries. Surficial sediment communities were different from each other and from the deep subsurface samples. Some fungal sequences represented potentially novel organisms as well as ones with a cosmopolitan distribution in terrestrial, fresh and salt water environments. Our results indicate that fungi are the most consistently detected eukaryotes in the marine sedimentary subsurface; further, some species may be specifically adapted to the deep subsurface and may play important roles in the utilization and recycling of nutrients.