Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9.
Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9.
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DOI：
10.20772/cancersci1985.78.1_54
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Iwamura;T. Katsuki;K. Ide
中科院分区：
文献类型：
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作者：
T. Iwamura;T. Katsuki;K. Ide
在组织培养和裸鼠体内建立了一株来源于人胰腺癌转移性肝癌的新的肿瘤细胞系(Suit-2)，并已维持了5年以上。在组织培养中，细胞生长在单层薄片中，群体倍增时间约为38.2小时，在相对融合的培养中，细胞漂浮或堆积在单层表面以上形成小芽。染色体数目在34到176之间，模式数为45。将培养的细胞皮下注射到裸鼠体内，导致肿瘤形成，组织病理学与原肿瘤非常相似，被归类为中分化管状腺癌。肿瘤细胞的电子显微镜观察显示有特征性的胰腺导管上皮。Suit-2细胞产生和释放至少两种肿瘤标志物，癌胚抗原和糖类抗原19-9，即使在无血清介质中也能增殖，并在裸鼠移植瘤中转移到区域淋巴结。
A new tumor cell line (SUIT-2) derived from a metastatic liver tumor of human pancreatic carcinoma has been established in tissue culture and in nude mice, and maintained for over five years. In tissue culture, the cells grew in a monolayered sheet with a population doubling time of about 38.2 hr, and floated or piled up to form small buds above the monolayered surface in relatively confluent cultures. Chromosome counts ranged from 34 to 176 with a modal number of 45. Subcutaneous injection of cultured cells into nude mice resulted in tumor formation, histopathologically closely resembling the original neoplasm which had been classified as moderately differentiated tubular adenocarcinoma. Electron microscopic observation of the neoplastic cells revealed a characteristic pancreatic ductal epithelium. SUIT-2 cell line produces and releases at least two tumor markers, carcinoembryonic antigen and carbohydrate antigen 19-9, propagates even in serum-free medium, and metastasizes to the regional lymph nodes in nude mice xenografts.