Characterization of a vasopressin-like peptide in rat and bovine blood vessels.
Characterization of a vasopressin-like peptide in rat and bovine blood vessels.
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大鼠和牛血管中加压素样肽的表征。
DOI：
10.1152/ajpheart.1992.262.3.h799
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kasson,BG
中科院分区：
文献类型：
--
作者：
Simon,JS;Brody,MJ;Kasson,BG
本研究探讨了大鼠和奶牛的血管中含有精氨酸加压素（AVP）的可能性，以及这种有效的血管收缩剂的血管储存是局部的，而不是血液传播的，起源。将大鼠或奶牛的血管在酸中匀浆，并通过放射免疫法测定上清液中的AVP。AVP免疫反应性检测在所有的血管取自大鼠，包括主动脉，肠系膜动脉，肾动脉，腔静脉，肾静脉，以及心房的心脏和牛的主动脉。垂体切除和Brattleboro大鼠的血管中也含有AVP，其水平与完整对照组大鼠相似。这种免疫反应性材料的进一步表征表明，在放射免疫分析和放射受体测定，血管提取物产生的竞争曲线，这是平行的合成AVP。此外，主动脉提取物中的免疫反应性AVP与合成AVP和垂体提取物在高压液相色谱和Sephadex G-25色谱上均发生共迁移。此外，静脉注射主动脉提取物在清醒大鼠产生升压反应，这些反应显着衰减预处理与特定的AVP V1受体拮抗剂。这些数据表明，血管含有一个AVP样肽，似乎无法区分从真正的AVP，并进一步表明，这种血管肽是本地的起源。
The present study examined the possibility that blood vessels of the rat and cow contain arginine vasopressin (AVP) and that the vascular stores of this potent vasoconstrictor are of local, rather than blood-borne, origin. Vessels from rat or cow were homogenized in acid, and the supernatants were assayed for AVP by radioimmunoassay. AVP immunoreactivity was detected in all vessels taken from the rat including aorta, mesenteric artery, renal artery, vena cava, and renal vein, as well as atria of the heart and also in cow aorta. Blood vessels from hypophysectomized and Brattleboro rats also contained AVP at levels similar to those of intact control rats. Further characterization of this immunoreactive material showed that, in both radioimmunoassay and radioreceptor assay, vascular extracts produced competition curves, which were parallel to those of synthetic AVP. Additionally, immunoreactive AVP in aortic extracts comigrated with synthetic AVP and pituitary extract on both high-pressure liquid chromatography and Sephadex G-25 chromatography. Furthermore, intravenous administration of the aortic extract produced pressor responses in conscious rats, and these responses were significantly attenuated by pretreatment with a specific AVP V1-receptor antagonist. These data demonstrate that blood vessels contain an AVP-like peptide that appears indistinguishable from authentic AVP and further suggest that this vascular peptide is of local origin.