Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell
Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell
复制标题
DOI：
10.1126/science.273.5272.242
复制
发表时间：
1996-07-12
期刊：
影响因子：
56.9
通讯作者：
Nakauchi, H
中科院分区：
文献类型：
--
作者：
Osawa, M;Hanada, K;Nakauchi, H
造血干细胞（HSCs）通过其自我更新和多向分化能力在整个生命过程中供应所有血细胞。使用针对CD 34的小鼠同源物（mCD 34）产生的单克隆抗体将小鼠HSC纯化至接近同质。与人类不同，在mCD 34低至阴性部分中检测到原始成年小鼠骨髓HSC。注射单个mCD 34（lo/-）、c-Kit（+）、Sca-1（+）、谱系标志物阴性（Lin（-））细胞导致21%的受者淋巴造血系统长期重建。因此，纯化的HSC群体应该能够分析个体HSC的自我更新和多谱系分化。
Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34(lo/-), c-Kit(+), Sca-1(+), lineage markers negative (Lin(-)) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.