A rapid method for high throughput DNA extraction from plant material for PCR amplification
A rapid method for high throughput DNA extraction from plant material for PCR amplification
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DOI：
10.1007/bf02825295
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发表时间：
2000-01-01
影响因子：
2.1
通讯作者：
Frey, JE
中科院分区：
文献类型：
--
作者：
Dilworth, E;Frey, JE
描述了一种快速、可靠的从植物材料中高通量提取DNA的方法，该方法使用玻璃微珠在平底微量滴定板中。该方法快速、廉价，最多可并行处理96个样品。回收的DNA产生的聚合酶链式反应产物与通过传统提取方法产生的产物一致。
A rapid and reliable method is described for high throughput extraction of DNA from plant material using glass beads in a fiat-bottomed microtitre plate. This: procedure is quick, inexpensive, and allows up to 96 samples to be processed in parallel. PCR products produced by the recovered DNA are consistently equivalent to those produced through traditional extraction methods.