A liquid chromatography-tandem mass spectrometry method for the detection of economically motivated adulteration in protein-containing foods
A liquid chromatography-tandem mass spectrometry method for the detection of economically motivated adulteration in protein-containing foods
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DOI：
10.1016/j.chroma.2011.11.066
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发表时间：
2012-01-13
影响因子：
4.1
通讯作者：
Stromgren, Selen A.
中科院分区：
文献类型：
--
作者：
Mac Mahon, Shaun;Begley, Timothy H.;Stromgren, Selen A.
开发了一种新的分析方法，以确定是否存在六（6）种可能用于经济掺假的化合物，以提高乳制品和散装蛋白质中的氮含量。用2%甲酸从基质中提取残留物，然后加入乙腈（ACN）诱导蛋白质沉淀。采用ZIC-HILIC色谱柱和串联质谱法（LC-MS/MS）（采用电喷雾电离（ESI）），通过液相色谱法分析浸提液。在浓度低至1.0 μ g/g（ppm）的6种强化基质中收集了单实验室方法验证数据。使用加标基质校准标准曲线的平均回收率和平均相对标准偏差（RSD）如下：环丙氨嗪（CY）95.9%（7.5% RSD），双氰胺（DC）98.1%尿素102.5%（8.6%），缩二脲97.2%（6.6%），三脲97.7%（5.7%），脒基脲（Au）93.4%（7.4%）。该方法提供了一种快速有效的方法，以积极打击含蛋白质产品中出于经济动机的掺假。由爱思唯尔公司出版
A new analytical method was developed to determine the presence of six (6) compounds with the potential to be used in economic adulteration to enhance the nitrogen content in milk products and bulk proteins. Residues were extracted from the matrix with 2% formic acid, after which acetonitrile (ACN) was added to induce precipitation of the proteins. Extracts were analyzed by liquid chromatography using a ZIC-HILIC column with tandem mass spectrometry (LC-MS/MS) using electrospray ionization (ESI). Single-laboratory method validation data was collected in six matrices fortified at concentrations down to 1.0 mu g/g (ppm). Average recoveries and average relative standard deviations (RSD) using spiked matrix calibration standard curves were the following: cyromazine (CY) 95.9% (7.5% RSD), dicyandiamide (DC) 98.1% (5.6% RSD), urea 102.5% (8.6% RSD), biuret (BU) 97.2% (6.6% RSD), triuret (TU) 97.7% (5.7% RSD), and amidinourea (AU) 93.4% (7.4% RSD). This method provides a rapid and effective approach to proactively combat economically motivated adulteration in protein-containing products. Published by Elsevier B.V.