IgE antibody to fish gelatin (type I collagen) in patients with fish allergy
IgE antibody to fish gelatin (type I collagen) in patients with fish allergy
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DOI：
10.1067/mai.2000.108499
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发表时间：
2000-09-01
影响因子：
14.2
通讯作者：
Inouye, S
中科院分区：
文献类型：
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作者：
Sakaguchi, M;Toda, M;Inouye, S
背景资料：大多数对麻疹、腮腺炎和风疹疫苗过敏的儿童对疫苗中含有的牛明胶表现出敏感性。近年来，研究发现明胶中主要含有的牛I型胶原蛋白是引起牛明胶过敏的主要过敏原，鱼肉和鱼皮中也含有牛I型胶原蛋白。（1）10例鱼类过敏和对鱼肉特异性IgE过敏的患者;（2）2例对鱼肉和牛明胶过敏和对鱼肉和牛明胶特异性IgE过敏的患者;（3）15例特应性皮炎和对鱼肉特异性IgE过敏的患者。从鱼皮中制备了各种鱼胶（I型胶原）。结果：10例鱼类过敏患者中，3例检测出针对鱼胶的特异性IgE。鱼过敏和牛明胶过敏的2例患者，都有特异性IgE的鱼明胶。在15例过敏性皮炎患者中，5例对鱼肉有特异性IgE，对鱼胶有特异性IgE。此外，从合并的患者血清中的IgE与鱼I型胶原蛋白的α 1和α 2链在免疫印迹中反应。结论：部分鱼源性过敏患者存在鱼源性明胶IgE抗体，但鱼源性明胶与牛源性明胶之间无交叉反应。鱼胶（I型胶原蛋白）可能是鱼类过敏受试者的过敏原。
Background: Most children with anaphylaxis to measles, mumps, and rubella vaccines had shown sensitivity to bovine gelatin that was included in the vaccines. Recently, it was found that bovine type I collagen, which is the main content in the gelatin, is a major allergen in bovine gelatin allergy, Fish meat and skin also contain type I collagen.Objective: The present study was designed to investigate IgE antibody to fish gelatin in children with fish allergy.Methods: Serum samples were taken from patients in 3 groups: (1) 10 patients with fish allergy and specific IgE to fish meat; (2) two patients with allergies to both fish meat and bovine gelatin and specific IgE to fish meat and bovine gelatin; and (3) 15 patients with atopic dermatitis and specific IgE to fish meat. Various fish gelatins (type I collagen) were prepared from fish skin. IgE antibody to fish gelatin was analyzed by using ELISA and immunoblotting.Results: Of 10 patients with fish allergy, 3 had specific IgE to fish gelatin. Of two patients with fish allergy and bovine gelatin allergy, all had specific IgE to fish gelatin. Of 15 patients with atopic dermatitis and specific IgE to fish meat, 5 had specific IgE to fish gelatin. Furthermore, IgE from pooled serum of the patients reacted with both the alpha 1 and alpha 2 chains of fish type I collagen in immunoblots. There is cross-reactivity among gelatins from various fishes, but there is little crossreactivity between fish and bovine gelatins.Conclusion: Some fish-sensitive patients possessed IgE antibody to fish gelatin. Fish gelatin (type I collagen) might be an allergen in subjects with fish allergy.