DIFFERENTIAL LETHAL EFFECTS OF BOTH CYTOSINE ARABINOSIDE AND HYDROXYUREA ON JEJUNAL CRYPT CELLS AND TESTOSTERONE‐STIMULATED ACCESSORY SEX GLANDS
DIFFERENTIAL LETHAL EFFECTS OF BOTH CYTOSINE ARABINOSIDE AND HYDROXYUREA ON JEJUNAL CRYPT CELLS AND TESTOSTERONE‐STIMULATED ACCESSORY SEX GLANDS
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阿糖胞苷和羟基脲对空肠隐窝细胞和睾酮刺激的副性腺的不同致死作用
DOI：
10.1111/j.1365-2184.1979.tb00170.x
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发表时间：
1979
影响因子：
8.5
通讯作者：
N. Wright
中科院分区：
文献类型：
--
作者：
M. Alison;N. Wright
小鼠空肠隐窝和副性腺的 DNA 合成细胞对 DNA 合成抑制剂阿糖胞苷和羟基脲的反应不同。单次注射任一试剂都会快速抑制两种组织中的胸苷标记。虽然这两种药物对隐窝中的大多数 S 期细胞具有致命作用，但副性腺中只有少数 S 期细胞显示出坏死的证据。这些差异是在持续分裂细胞和假定的 G0 细胞之间可能存在的生理差异的背景下考虑的。副性腺通常是静止的增殖组织。阉割后三天开始，通过丙酸睾酮注射刺激它们进行 DNA 合成和随后的有丝分裂。阿糖胞苷是副性腺中更有效的杀细胞剂，当安排两次注射以影响大量 DNA 合成细胞时，会引起代偿性增生。在凝血腺中，这种代偿反应涉及许多细胞的增殖，在没有细胞毒性扰动的情况下，这些细胞将不增殖（Q 细胞）。
DNA‐synthesizing cells from mouse jejunal crypts and accessory sex glands respond differently to the DNA synthesis inhibitors cytosine arabinoside and hydroxyurea. A single injection of either agent brought about a rapid inhibition of thymidine labelling in both the tissues. Whilst both agents had a lethal effect upon the majority of S‐phase cells in the crypts, only a minority of S‐phase cells in the accessory sex glands showed evidence of necrosis. These differences are considered in the context of possible physiological differences between continually dividing cells and putative G0 cells. The accessory sex glands are normally quiescent proliferative tissues. They were stimulated to undergo DNA synthesis and later mitosis by testosterone propionate injections, commencing three days after castration. Cytosine arabinoside was the more effective cytocidal agent in the accessory sex glands, and when two injections were scheduled so as to affect a large number of DNA‐synthesizing cells, a compensatory hyperplasia was evoked. In the coagulating gland, this compensatory response involved the proliferation of many cells which, in the absence of cytotoxic perturbation, would be non‐proliferative (Q cells).