Genetic dissection of rice (Oryza sativa L.) tiller, plant height, and grain yield based on QTL mapping and metaanalysis
Genetic dissection of rice (Oryza sativa L.) tiller, plant height, and grain yield based on QTL mapping and metaanalysis
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基于 QTL 作图和荟萃分析的水稻 (Oryza sativa L.) 分蘖、株高和籽粒产量的遗传解析
DOI：
10.1007/s10681-018-2187-2
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发表时间：
2018-07-01
期刊：
影响因子：
1.9
通讯作者：
Zou, D. T.
中科院分区：
文献类型：
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作者：
Lei, L.;Zheng, H. L.;Zou, D. T.
单株分蘖数和株高是与水稻产量相关的重要农艺性状。共检测到30个加性数量性状位点（A-QTL）和9个显著的加性×环境互作QTL（AE-QTL），表型相关和QTL相关分析证实了3个性状间的内在联系。通过Meta分析，将这些QTL与已有的986个QTL进行整合。共有图谱包含7156个标记，总图谱长度为1112.71 cM，共检测到863个QTL，其中78个元QTL（MQTLs）覆盖了30个QTL中的11个。在物理长度< 0.3 Mb的21个QTL区间上共检测到705个预测基因，筛选出13个与产量和植株发育相关的MQTL和34个候选基因。其中qGY 6 -2、qPH 7 -2、qPH 6 -3、qTN 6 -1和qTN 7 -1 5个主效QTL在QTL区间内未检测到，可作为新发现的QTL。这些QTL区间内的候选基因将在水稻TN、PH和戈伊的分子标记辅助选择和图位克隆中发挥有意义的作用。
Tiller number per plant (TN) and plant height (PH) are important agronomic traits related to grain yield (GY) in rice (Oryza sativa L.). A total of 30 additive quantitative trait loci (A-QTL) and 9 significant additive x environment interaction QTLs (AE-QTL) were detected, while the phenotypic and QTL correlations confirmed the intrinsic relationship of the three traits. These QTLs were integrated with 986 QTLs from previous studies by metaanalysis. Consensus maps contained 7156 markers for a total map length of 1112.71 cM, onto which 863 QTLs were projected; 78 meta-QTLs (MQTLs) covering 11 of the 30 QTLs were detected from the cross between Dongnong422 and Kongyu131 in this study. A total of 705 predicted genes were distributed over the 21 MQTL intervals with physical length < 0.3 Mb; 13 of the 21 MQTLs, and 34 candidate genes related to grain yield and plant development, were screened. Five major QTLs, viz. qGY6-2, qPH7-2, qPH6-3, qTN6-1, and qTN7-1, were not detected in the MQTL intervals and could be used as newly discovered QTLs. Candidate genes within these QTL intervals will play a meaningful role in molecular marker-assisted selection and map-based cloning of rice TN, PH, and GY.