Ovarian steroids modulate the self-priming effect of luteinizing hormone-releasing hormone on bovine pituitary cells in vitro.
Ovarian steroids modulate the self-priming effect of luteinizing hormone-releasing hormone on bovine pituitary cells in vitro.
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卵巢类固醇在体外调节黄体生成素释放激素对牛垂体细胞的自启动作用。
DOI：
10.1210/endo-110-3-717
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
E. Convey
中科院分区：
文献类型：
--
作者：
V. Padmanabhan;K. Leung;E. Convey
LHRH有能力启动脑下垂体前叶，从而增加标准剂量的LHRH释放的黄体生成素和卵泡刺激素的数量。这种启动效应可能对黄体生成素的分泌起着重要的调节作用。在这里描述的实验中，我们证明了LHRH的启动是通过对牛促性腺激素细胞的直接作用而发生的，并且LHRH的启动受卵巢类固醇的调节。牛脑垂体细胞按如下方式预置：0次换液，然后每隔50分钟换液400分钟。在每次换液前10分钟加入LHRH，即细胞在连续8次50分钟的孵育中暴露于LHRH 10分钟。在400min结束时，将垂体细胞与0或1 ng/mlLHRH孵育4h(试验期)，以评估预充程度。第一个被测试的变量是LHRH的预备量(0.001、0.1、0.1或1 ng/ml)。一般来说，促黄体生成素释放不影响黄体生成素的基础释放，但确实增加了试验剂量的促黄体生成素释放的能力。举个例子。
LHRH has the ability to prime the anterior pituitary gland, thereby increasing the quantity of LH and FSH released by a standard dose of LHRH. This priming effect may play an important regulatory role in LH secretion. In experiments described herein, we demonstrate that LHRH priming occurs via a direct effect on bovine gonadotrophs and LHRH priming is modulated by ovarian steroids. Bovine pituitary cells were primed as follows: Medium was changed at time zero, then at 50-min intervals for 400 min. LHRH was added 10 min before each medium change, i.e. cells were exposed to LHRH for 10 min during each of eight consecutive 50-min incubations. At the end of this 400 min, the degree of priming was assessed by incubating the pituitary cells with 0 or 1 ng/ml LHRH for 4 h (test period). The first variable tested was priming dose of LHRH (0, 0.001, 0.01,0.1, or 1 ng/ml). In general, LHRH priming did not affect basal release of LH but did increase the ability of a test dose of LHRH to induce LH release. For exampl...
使用来自卵巢切除、类固醇引发的大鼠的下丘脑内侧基底的推拉插管估计黄体生成素释放激素的体内释放。
影响因子：
4.8
作者：
Levine,JE;Ramirez,VD
通讯作者：
Ramirez,VD