Clinical significance of antibodies to bovine and human thrombin and factor V after surgical use of bovine thrombin.
Clinical significance of antibodies to bovine and human thrombin and factor V after surgical use of bovine thrombin.
复制标题
手术使用牛凝血酶后牛和人凝血酶和 V 因子抗体的临床意义。
DOI：
10.1093/ajcp/97.1.84
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发表时间：
1992
影响因子：
3.5
通讯作者：
Donnelly,K
中科院分区：
文献类型：
--
作者：
Rapaport,SI;Zivelin,A;Minow,RA;Hunter,CS;Donnelly,K
两名在手术期间接触牛凝血酶的患者产生了与牛和人凝血酶以及因子 V 发生反应的抗体。他们的凝血酶时间在使用牛凝血酶时显着延长，而在使用人凝血酶时则适度延长。高滴度抗牛因子 V 通过中和凝血酶原测定底物中的牛因子 V 造成一名患者的诊断混乱。尽管较弱，但针对人 V 因子的抗体活性导致两名患者术后 V 因子缺乏。这种交叉反应抗体可通过其针对牛的滴度高于人因子 V 来识别，当手术暴露于牛凝血酶的患者在手术后产生因子 V 抗凝剂时，应怀疑这种交叉反应抗体。吸附的牛血浆与作为抗体的每位患者血浆的交叉免疫电泳显示许多沉淀素弧，表明患者对商业凝血酶制剂中的其他蛋白质进行了免疫。
Two patients exposed during surgery to bovine thrombin developed antibodies reacting with both bovine and human thrombin and Factor V. Their thrombin times were markedly prolonged with bovine thrombin and modestly prolonged with human thrombin. High titer anti-bovine Factor V created diagnostic confusion in one patient by neutralizing bovine Factor V in a prothrombin assay substrate. Although weaker, antibody activity against human Factor V led to postoperative factor V deficiency in both patients. Such cross-reacting antibodies, recognizable by their higher titer against bovine than human Factor V, should be suspected when a patient surgically exposed to bovine thrombin develops a Factor V anticoagulant after operation. Crossed immunoelectrophoresis of adsorbed bovine plasma with each patient’s plasma as antibody revealed many precipitin arcs indicative of immunization of the patients to additional proteins in a commercial thrombin preparation.