AN ANIMAL-MODEL FOR THE ASSESSMENT OF GINGIVAL LESIONS
AN ANIMAL-MODEL FOR THE ASSESSMENT OF GINGIVAL LESIONS
复制标题
DOI：
10.1016/1056-8719(94)90070-1
复制
发表时间：
1994-11-01
影响因子：
1.9
通讯作者：
ROCHARVEILLER, M
中科院分区：
文献类型：
--
作者：
GUESSOUS, F;HUYNH, C;ROCHARVEILLER, M
在体重180-200 g的Sprague-Dawley大鼠中进行牙龈炎症模型。当竹杆被细菌污染时，机械竹杆引起的损伤是炎症性的。细菌首先从收集自成人牙周炎患者的牙龈液中获得。巴斯德研究所的另一种菌株（IP 6444）诱导了类似的炎症。10天后，通过在牙龈提取物中进行弹性蛋白酶测定来定量炎症。同时，观察弹性结构，并通过自动图像分析对弹性纤维进行定量。这种“嵌塞”技术能够诱导牙龈炎症反应，其特征在于牙龈弹性蛋白酶含量显著增加、引发的细胞浸润牙龈组织和牙龈弹性纤维断裂。这些参数是相关的，其中之一的测量可能是有用的药理学研究应用于治疗牙周病变。显示了从肝素处理的动物中获得的结果的示例。
A model of gingival inflammation was performed in Sprague-Dawley rats weighing 180-200 g. Mechanical bamboo stick-induced injury was inflammatory when bacteria contaminated the sticks. Bacteria were first obtained from gingival fluid collected from a patient with adult periodontitis. Another strain from Institut Pasteur (IP 6444) induced similar inflammation. Inflammation was then quantified 10 days later by means of elastase assays performed in gingival extracts. In parallel, elastic structures were observed and elastic fibers were quantified by automated image analysis. This technique of ''impaction'' was able to induce a gingival inflammatory reaction characterized by a significant increase of gingival elastase content, infiltration of gingival tissues by elicited cells, and gingival elastic fiber breakdown. These parameters were correlated, and measurement of one of them might be useful for pharmacological studies applied to the treatment of periodontal lesions. An example of results obtained from animals treated by heparine was shown.