INHIBITION OF NEUTROPHIL OXIDANT SECRETION BY D-PENICILLAMINE - SCAVENGING OF H2O2 AND HOCL
INHIBITION OF NEUTROPHIL OXIDANT SECRETION BY D-PENICILLAMINE - SCAVENGING OF H2O2 AND HOCL
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DOI：
10.1136/ard.51.3.321
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发表时间：
1992-03-01
影响因子：
27.4
通讯作者：
EDWARDS, SW
中科院分区：
文献类型：
--
作者：
LEDSON, MJ;BUCKNALL, RC;EDWARDS, SW
用鲁米诺依赖化学发光法测定，D-青霉胺可抑制中性粒细胞经趋化肽N-甲酰甲硫基亮氨酰苯丙氨酸(fMet-Leu-Phe)激活后的氧化剂分泌。相反，这种药物对细胞内氧化剂的产生或同一激动剂激活的荧光素依赖的化学发光几乎没有影响。在无细胞鲁米诺化学发光体系中，该药物对H_2O_2和HOCl均有清除作用，但其清除H_2O_2的能力强于H_2O_2。这两种氧化剂都可以氧化药物，但HOCl2的效力又一次高于双氧水。当D-青霉胺被暴露在过氧化氢中氧化时，它不能再作为中性粒细胞分泌的氧化剂的清除剂。这些数据表明，在体内，在分泌髓过氧化物酶可能起作用的病理条件下，HOCl的优先清除可能是重要的。
D-Penicillamine inhibited oxidant secretion from human neutrophils after activation by the chemotactic peptide N-formyl-methionylleucyl-phenylalanine (fMet-Leu-Phe), as assessed by luminol dependent chemiluminescence. In contrast, this drug had little effect on either intracellular oxidant production or lucigenin dependent chemiluminescence activated by the same agonist. The drug was shown to scavenge both H2O2 and HOCl in a cell free luminol chemiluminescence system, though its ability to scavenge HOCl was greater than that for H2O2. Both these oxidants could oxidise the drug, but again HOCl was more potent than H2O2. When D-penicillamine was oxidised by exposure to H2O2 it could no longer serve as a scavenger of secreted oxidants from neutrophils. These data suggest that in vivo the preferential scavenging of HOCl may be important under pathological conditions where secreted myeloperoxidase may be functional.