Simultaneous quantification of malonyl-CoA and several other short-chain acyl-CoAs in animal tissues by ion-pairing reversed-phase HPLC/MS
Simultaneous quantification of malonyl-CoA and several other short-chain acyl-CoAs in animal tissues by ion-pairing reversed-phase HPLC/MS
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DOI：
10.1016/j.jchromb.2007.03.029
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发表时间：
2007-06-15
影响因子：
3
通讯作者：
Burns, David J.
中科院分区：
文献类型：
--
作者：
Gao, Lan;Chiou, William;Burns, David J.
丙二酰辅酶a是参与脂质合成和脂质氧化的关键中间体。在这里，我们报告了一种新的方法，用于定量丙二酰辅酶a和其他七种短链酰基辅酶a在各种大鼠和小鼠组织使用离子配对反相高效液相色谱/质谱。该方法可同时测定丙二酰辅酶A、游离辅酶A （CoASH）、乙酰-辅酶A、β -羟基-丁基-辅酶A （HB-CoA）、3-羟基-3-甲基-戊二酰辅酶A （HMG-CoA）、丙炔-辅酶A、琥珀酰辅酶A和异丁基-辅酶A， HPLC梯度7.0 min，动态线性范围超过两个数量级。除HMG-CoA的定量下限为0.90 pmol外，所有酰基辅酶a的定量下限均为0.225 pmol。首次探讨了HB-CoA对动物组织中丙二酰辅酶a定量的干扰。(c) 2007 Elsevier B.V.版权所有
Malonyl-CoA is a key intermediate involved in lipid synthesis and lipid oxidation. Here, we report on a novel method for the quantification of malonyl-CoA and seven other short-chainacyl-CoAs in various rat and mouse tissues using ion-pairing reversed-phase HPLC/MS. This method is capable of measuring malonyl-CoA, free coenzyme A (CoASH), acetyl-CoA, beta-hydroxyl-butyryi-CoA (HB-CoA), 3-hydroxy-3-methyl-glutarylCoA (HMG-CoA), propionyl-CoA, succinyl-CoA, and isobutyryl-CoA simultaneously with a dynamic linear range over two orders of magnitude in a 7.0 min HPLC gradient run. The lower limit of quantification (LLOQ) was 0.225 pmol for all acyl-CoAs studied, except for HMG-CoA which had a higher LLOQ of 0.90 pmol. The interference of HB-CoA on the quantification of malonyl-CoA in animal tissues was also explored for the first time. (c) 2007 Elsevier B.V. All rights reserved.