Oral hypoglycaemic, antihyperglycaemic and antidiabetic activities of Sri Lankan Broken Orange Pekoe Fannings (BOPF) grade black tea (Camellia sinensis L.) in rats
Oral hypoglycaemic, antihyperglycaemic and antidiabetic activities of Sri Lankan Broken Orange Pekoe Fannings (BOPF) grade black tea (Camellia sinensis L.) in rats
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DOI：
10.1016/j.jep.2011.02.035
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发表时间：
2011-05-17
影响因子：
5.4
通讯作者：
Amarakoon, A. M. T.
中科院分区：
文献类型：
--
作者：
Abeywickrama, K. R. W.;Ratnasooriya, W. D.;Amarakoon, A. M. T.
民族药物学相关性：斯里兰卡传统从业者建议食用由茶树L植物制成的红茶冲泡液来调节血糖。然而，他们没有具体说明茶叶的等级及其原产地(即农业气候海拔)，因此许多糖尿病前期患者和轻度糖尿病患者使用BOPF级茶。研究目的：本研究检验斯里兰卡BOPF级茶的降血糖潜力及其相对于农业气候海拔的效力。材料和方法：未混合的正统BOPF级茶样品来自斯里兰卡高、中、低种植的农业气候海拔。对不同剂量的温血BTI(60、120、480 mg/ml)、甲苯磺丁胺(参比药物：22.5 mg/kg体重)和水(对照组)进行灌胃，评价其降血糖和降血糖作用。用链脲佐菌素诱导的糖尿病大鼠测定其抗糖尿病活性。结果：BTI表现出显著的(P<；0.05)、剂量依赖和显著的降血糖和降血糖活性，且起效快。这些影响在农业气候海拔方面没有不同，尽管植物成分的含量有所不同。BTI还显示出显著且快速的抗糖尿病活性。BTI抑制肠道葡萄糖吸收，降低α-葡萄糖苷酶和α-淀粉酶活性。BTI具有胰岛素样作用、提高胰岛素敏感性和体内抗氧化活性。结论：斯里兰卡BOPF级茶叶的BTI具有口服降血糖、降血糖和降血糖作用，其作用机制是多方面的。本研究还表明，斯里兰卡任何农业气候海拔的BOPF级茶都可以用于调节血糖。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
Ethnopharmcological relevance: Sri Lankan traditional practitioners recommend the consumption of black tea infusion (BTI) made from Camellia sinensis L plant for regulation of glycaemia. However, they do not specify the grade of tea and their origin (i.e., agroclimatic elevation) and as such many prediabetics and milddiabetics use BOPF grade tea.Aim of the study: This study examines the blood glucose lowering potential of Sri Lankan BOPF grade tea and its potency with respect to agroclimatic elevations.Materials and methods: Unblended orthodox BOPF grade tea samples were collected from high-, mid- and low-grown agroclimatic elevations in Sri Lanka. Different concentrations of warm BTI (60, 120 and 480 mg/ml), tolbutamide (reference drug: 22.5 mg/kg body weight) and water (control) were orally administered to different groups of rats, and hypoglycaemic and antihyperglycaemic activities were assessed. Antidiabetic activity was determined using streptozotocin induced diabetic rats. Mechanisms of blood glucose lowering actions were investigated using several standards techniques.Results: BTI exhibited significant (P < 0.05), dose-dependent and marked hypoglycaemic and antihyperglycaemic activities with quick onset. These effects did not differ with respect to agroclimatic elevation, although there were differences in the content of phyto-constituents. BTI also showed marked and quick antidiabetic activity. BTI inhibited intestinal glucose absorption and impaired alpha-glucosidase and alpha-amylase activities. BTI possessed insulinomimetic action, ability to improve insulin sensitivity and in vivo antioxidant activity. Notably, BTI was nontoxic.Conclusions: BTI of Sri Lankan BOPF grade tea has oral hypoglycaemic, antihyperglycaemic and antidiabetic actions which are mediated via multiple mechanisms. This study also indicates that, BOPF grade tea of any agroclimatic elevations in Sri Lanka could be used in the regulation of glycaemia. (C) 2011 Elsevier Ireland Ltd. All rights reserved.