Microsatellite DNA polymorphism and heterozygosity among field and laboratory populations of Anopheles gambiae s.s. (Diptera: Culicidae)
Microsatellite DNA polymorphism and heterozygosity among field and laboratory populations of Anopheles gambiae s.s. (Diptera: Culicidae)
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DOI：
10.1603/0022-2585-38.2.336
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发表时间：
2001-03-01
影响因子：
2.1
通讯作者：
Lanzaro, GC
中科院分区：
文献类型：
--
作者：
Norris, DE;Shurtleff, AC;Lanzaro, GC
我们比较了两个菌落中的九个基因座的微卫星多态性，在两个菌落中，冈比亚sensu sensu sensu sermu giles蚊子的田间种群。在每个基因座观察到的微卫星等位基因的数量和平均杂合子在实验室菌落之间大大减少。对本研究中使用的现场种群的遗传分析显示，稀有等位基因的前所未有的频率（0.50。对野外数据进行分配，以评估稀有等位基因，无效等位基因和样本量对平均杂合性估计值的影响揭示了这种测量和测量的可塑性表明可以从使用微卫星相对较小的集合可靠地估算杂合性。
We compared microsatellite polymorphism at nine loci located on chromosome 3 among two colonies and a field population of Anopheles gambiae sensu stricto Giles mosquitoes. Numbers of microsatellite alleles observed at each locus and mean heterozygosities were drastically reduced among laboratory colonies. Genetic analysis of the field population used in this study revealed an unprecedented frequency of rare alleles (0.50. Partitioning of field data to assess the impact of rare alleles, null alleles, and sample size on estimates of mean heterozygosity revealed the plasticity of this measurement and suggests that heterozygosity may be reliably estimated from relatively small collections using microsatellites.