Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro.
Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro.
复制标题
体外人 MCF-7/S 和 MCF-7/ADR 细胞中超声靶向微泡破坏介导 siRNA 转染增强 PEAL 纳米颗粒的递送
DOI：
10.2147/ijn.s81172
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发表时间：
2015
影响因子：
8
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
Teng Y;Bai M;Sun Y;Wang Q;Li F;Xing J;Du L;Gong T;Duan Y
小干扰 RNA (siRNA) 的基因敲除活性使其成为多种疾病的潜在治疗方法。然而，成功的基因治疗需要安全有效的递送系统。在本研究中，我们选择 mPEG-PLGA-PLL 纳米粒子 (PEAL NPs) 与超声靶向微泡破坏 (UTMD) 来有效地将 siRNA 递送到细胞中。采用乳化-溶剂蒸发法制备负载siRNA的PEAL NPs。纳米粒子平均尺寸为132.6±10.3 nm（n=5），形状均匀，球形，包封率（EE）大于98%。 MTT 测定表明，即使在高浓度下，PEAL NP 和负载 siRNA 的 PEAL NP 均未表现出细胞毒性。细胞摄取结果表明，在UTMD的帮助下，负载siRNA的PEAL NPs可以有效地内化，并随后在细胞中释放siRNA。总而言之，带有 UTMD 的 PEAL NP 在 siRNA 递送方面可能非常有前景，从而可以充分利用基于 siRNA 的疗法的潜力。
The gene knockdown activity of small interfering RNA (siRNA) has led to their use as potential therapeutics for a variety of diseases. However, successful gene therapy requires safe and efficient delivery systems. In this study, we choose mPEG-PLGA-PLL nanoparticles (PEAL NPs) with ultrasound targeted microbubble destruction (UTMD) to efficiently deliver siRNA into cells. An emulsification-solvent evaporation method was used to prepare siRNA-loaded PEAL NPs. The NPs possessed an average size of 132.6±10.3 nm (n=5), with a uniform spherical shape, and had an encapsulation efficiency (EE) of more than 98%. As demonstrated by MTT assay, neither PEAL NPs nor siRNA-loaded PEAL NPs showed cytotoxicity even at high concentrations. The results of cellular uptake showed, with the assistance of UTMD, the siRNA-loaded PEAL NPs can be effectively internalized and can subsequently release siRNA in cells. Taken together, PEAL NPs with UTMD may be highly promising for siRNA delivery, making it possible to fully exploit the potential of siRNA-based therapeutics.