ROLE OF NITRIC-OXIDE IN NMDA-EVOKED RELEASE OF [H-3] DOPAMINE FROM STRIATAL SLICES
ROLE OF NITRIC-OXIDE IN NMDA-EVOKED RELEASE OF [H-3] DOPAMINE FROM STRIATAL SLICES
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DOI：
10.1097/00001756-199205000-00008
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发表时间：
1992-05-01
期刊：
影响因子：
1.7
通讯作者：
GALLY, JA
中科院分区：
文献类型：
--
作者：
HANBAUER, I;WINK, D;GALLY, JA
有证据表明，兴奋性氨基酸通过n -甲基- d -天冬氨酸（NMDA）受体起作用，激发儿茶酚胺从轴突终末和突触体释放，这被用来证明突触前NMDA受体的存在。NMDA受体激动剂也产生一氧化氮（NO），其迅速扩散通过神经组织。我们发现外源性NO诱导培养神经元释放[H-3]-多巴胺。这种释放不被NMDA拮抗剂MK-801和河豚毒素阻断。抑制NO合成的N(G)-硝基精氨酸和结合细胞外NO的血红蛋白都能阻断nmda诱导的纹状体片释放[H-3]-多巴胺。内源性合成一氧化氮的一个主要作用可能是引起神经组织局部体积的神经递质释放。
EVIDENCE that excitatory amino acids act via N-methyl-D-aspartate (NMDA) receptors to evoke the release of catecholamines from axonal terminals and synaptosomes has been used to argue for the presence of pre-synaptic NMDA receptors. NMDA receptor agonists also generate nitric oxide (NO) which rapidly diffuses through neural tissue. We find that exogenously applied NO evokes [H-3]-dopamine release from cultured neurons. This release is not blocked by the NMDA antagonist MK-801 nor by tetrodotoxin. Both N(G)-nitroarginine which inhibits NO synthesis, and hemoglobin which binds extracellular NO, block NMDA-evoked [H-3]-dopamine release from striatal slices. A major role of endogenously-synthesized NO may be to evoke neurotransmitter release in local volumes of neural tissue.