KAR3-ENCODED KINESIN IS A MINUS-END-DIRECTED MOTOR THAT FUNCTIONS WITH CENTROMERE-BINDING PROTEINS (CBF3) ON AN IN-VITRO YEAST KINETOCHORE
KAR3-ENCODED KINESIN IS A MINUS-END-DIRECTED MOTOR THAT FUNCTIONS WITH CENTROMERE-BINDING PROTEINS (CBF3) ON AN IN-VITRO YEAST KINETOCHORE
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DOI：
10.1073/pnas.91.15.7212
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发表时间：
1994-07-19
影响因子：
11.1
通讯作者：
CARBON, J
中科院分区：
文献类型：
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作者：
MIDDLETON, K;CARBON, J
我们已经使用体外运动试验，以探讨在芽殖酵母酿酒酵母动粒功能的机制。功能着丝粒DNA加上一个三方着丝粒结合蛋白复合物，CBF3，被认为是必要的，但不足以在体外动粒活性。第四个必要的组成部分被确定为马达蛋白Kar3p，以前报道的酵母驱动蛋白已知参与核配和有丝分裂。我们的数据支持遗传学证据表明，Kar3p是一个kinetochore相关的电机，并暗示CBF3起着调节作用的动粒功能。
We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth required component was identified as the motor protein Kar3p, a previously reported yeast kinesin known to be involved in karyogamy and mitosis. Our data support genetic evidence suggesting that Kar3p is a kinetochore-associated motor and imply that CBF3 plays a regulatory role in kinetochore function.